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Structural determin...
Structural determination of the O-antigenic polysaccharide from Escherichia coli O74
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- Jonsson, K. Hanna M. (author)
- Stockholms universitet,Institutionen för organisk kemi
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- Weintraub, Andrej (author)
- Karolinska Institutet
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- Widmalm, Göran (author)
- Stockholms universitet,Institutionen för organisk kemi
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(creator_code:org_t)
- Elsevier Ltd. 2009
- 2009
- English.
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In: Carbohydrate Research. - : Elsevier Ltd.. - 0008-6215 .- 1873-426X. ; 344:12, s. 1592-1595
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The structure of the O-antigen polysaccharide (PS) from Escherichia coli O74 has been determined. Component analysis, together with 1H and 13C NMR spectroscopy as well as 1H,15N-HSQC experiments were employed to elucidate the structure. Inter-residue correlations were determined by 1H,1H-NOESY and 1H,13C-heteronuclear multiple-bond correlation experiments. The PS is composed of tetrasaccharide repeating units with the following structure: Full-size image (5K) Cross-peaks of low intensity from an α-linked N-acetylglucosamine residue were present in the NMR spectra, and spectral analysis indicates that they originate from the penultimate residue in the polysaccharide. Consequently, the biological repeating unit has a 3-substituted N-acetyl-d-glucosamine residue at its reducing end. The 1H, 13C and 15N NMR chemical shifts of the α- and β-anomeric forms of d-Fucp3NAc are also reported. The repeating unit of the E. coli O74 O-antigen is identical to that of the capsular polysaccharide from E. coli K45.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Other Basic Medicine (hsv//eng)
Keyword
- Escherichia coli; Lipopolysaccharide; Capsular polysaccharide; NMR; Biological repeating unit
- Chemistry
- Kemi
- organisk kemi
- Organic Chemistry
Publication and Content Type
- ref (subject category)
- art (subject category)
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