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A Mycobacterium tub...
A Mycobacterium tuberculosis ligand-binding Mn/Fe protein reveals a new cofactor in a remodeled R2-protein scaffold
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- Andersson, Charlotta S (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Högbom, Martin (author)
- Uppsala universitet,Stockholms universitet,Institutionen för biokemi och biofysik,Institutionen för cell- och molekylärbiologi
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(creator_code:org_t)
- 2009-04-07
- 2009
- English.
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490 .- 0027-8424. ; 106:14, s. 5633-8
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Abstract
Subject headings
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- Chlamydia trachomatis R2c is the prototype for a recently discovered group of ribonucleotide reductase R2 proteins that use a heterodinuclear Mn/Fe redox cofactor for radical generation and storage. Here, we show that the Mycobacterium tuberculosis protein Rv0233, an R2 homologue and a potential virulence factor, contains the heterodinuclear manganese/iron-carboxylate cofactor but displays a drastic remodeling of the R2 protein scaffold into a ligand-binding oxidase. The first structural characterization of the heterodinuclear cofactor shows that the site is highly specific for manganese and iron in their respective positions despite a symmetric arrangement of coordinating residues. In this protein scaffold, the Mn/Fe cofactor supports potent 2-electron oxidations as revealed by an unprecedented tyrosine-valine crosslink in the active site. This wolf in sheep's clothing defines a distinct functional group among R2 homologues and may represent a structural and functional counterpart of the evolutionary ancestor of R2s and bacterial multicomponent monooxygenases.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- bioinorganic chemistry
- diiron
- manganese
- monooxygenase
- R2c
- NATURAL SCIENCES
- NATURVETENSKAP
- Biophysics
- biofysik
- Biochemistry
- biokemi
- Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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