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Acrylamide-induced ...
Acrylamide-induced effects on general and neurospecific cellular functions during exposure and recovery.
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- Nordin-Andersson, M (author)
- Stockholms universitet,Institutionen för neurokemi
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Walum, E (author)
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Kjellstrand, P (author)
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- Forsby, A (author)
- Stockholms universitet,Institutionen för neurokemi
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(creator_code:org_t)
- 2003
- 2003
- English.
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In: Cell Biology and Toxicology. - 0742-2091 .- 1573-6822. ; 19:1, s. 43-51
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- Basal cytotoxicity, morphological changes and alterations in cell physiological and neurochemical functions were studied in differentiated human neuroblastoma (SH-SY5Y) cells during exposure to acrylamide and during a subsequent recovery period after cessation of exposure. Acrylamide induced a 20% reduction in the number of neurites per cell at 0.21 mmol/L and 20% decrease in the protein synthesis rate at 0.17 mmol/L after 72 h of exposure. Furthermore, the basal level of intracellular calcium concentration ([Ca2+]i) and receptor-activated (carbachol, 0.1 mmol/L) Ca2+ fluxes increased by 49% and 21%, respectively, at 0.25 mmol/L. These observations were made at noncytotoxic acrylamide concentrations, signifying specific neurotoxic alterations. Forty-eight hours after cessation of acrylamide exposure, the SH-SY5Y cells had recovered, i.e., the number of neurites per cell as well as the basal level of [Ca2+]i and rate of protein synthesis were comparable to those of control cells. The general calpain inhibitor calpeptin decreased the acrylamide-induced (0.5 mmol/L) neurite degeneration, determined as reduction in number of neurites per cell, from 52% to 17% as compared to control cells, which further supports the hypothesis that an increased [Ca2+]i plays a significant role for acrylamide-induced axonopathy.
Keyword
- NATURAL SCIENCES
- NATURVETENSKAP
- Neurochemistry and Molecular Neurobiology
- neurokemi med molekylär neurobiologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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