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Differential neuron...
Differential neuronal expression of three Drosophila ion transport peptide splice forms indicate multiple functions of peptidergic neurons
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- Dircksen, Heinrich (author)
- Stockholms universitet,Avdelningen för funktionell zoomorfologi,Zoologiska institutionen,Dircksen, Heinrich
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- Mandali, Aditya (author)
- Stockholms universitet,Avdelningen för funktionell zoomorfologi,Zoologiska institutionen
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Yoshii, Taishi (author)
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- Strauss, Johannes (author)
- Stockholms universitet,Avdelningen för funktionell zoomorfologi,Zoologiska institutionen
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Helfrich-Foerster, Charlotte (author)
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- Nässel, Dick R (author)
- Stockholms universitet,Avdelningen för funktionell zoomorfologi,Zoologiska institutionen
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
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In: Comparative Biochemistry and Physiology A. - : Elsevier BV. - 1095-6433 .- 1531-4332. ; 153A:2, suppl. 1, s. S79-
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- We identified previously two long (DrmITPL1 and -L2) and one amidated short isoform (DrmITP) of insect ion transport peptides (ITPs) as products derived by alternatively splicing from the Drosophila itp-gene (CG13586). The peptides are members of a large family of arthropod neuropeptides incl. crustacean hyperglycemic hormones (CHH/ITP-family), but similar ITPs are only known in locusts to have antidiuretic bioactivity on the hindgut. We localised the peptides by in situ hybridisation and immunocytochemistry with isoform-specific antibodies in the nervous system of larval (L3) and adult Drosophila melanogaster and screened Gal4-lines specific for peptidergic cells. Four neurosecretory cells in brain-corpora cardiaca/allata putatively release DrmITP as a hormone in all stages. DrmITP also occurs in interneurons in the brain/ventral ganglia and in neurons efferent towards the hindgut. Some interneurons are identical to well-known circadian clock neurons for which the effector molecules were elusive but are responsible for the evening bouts of locomotor activity in flies. DrmITPL1 and -L2 were found only in peripheral lateral bipolar and putative sensory neurons which are likely to play a role in the control of growth, hindgut ion transport and heart beat. With DrmITP identified in brain neurosecretory cells, hindgut-innervating neurons in the abdominal ganglia and one pair in the abdomen close to the larval anal organ or innervating the adult rectal pads, both chloride-transporting organs, we are facing an enormous complexity in multiple functions of differentially expressed ITP/Ls derived from a single gene. Preliminary results using Gal4-driven RNAi in distinct peptidergic neurons look promising to find deficiency phenotypes.
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