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The exosome Associa...
The exosome Associates Cotranscriptionally with the Nascent Pre-mRNP through Interactions with Heterogeneous Nuclear Ribonucleoproteins
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- Hessle, Viktoria (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Björk, Petra (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Sokolowski, Marcus (author)
- Karolinska Institutet,Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Gonzalez de Valdivia, Ernesto (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Silverstein, Rebecca (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Artemenko, Konstantin (author)
- Uppsala universitet,Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik,Institutionen för cell- och molekylärbiologi
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- Tyagi, Anu (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Maddalo, Gianluca (author)
- Stockholms universitet,Institutionen för analytisk kemi
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- Ilag, Leopold (author)
- Stockholms universitet,Institutionen för analytisk kemi
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- Helbig, Roger (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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- Zubarev, Roman A. (author)
- Karolinska Institutet,Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Visa, Neus (author)
- Stockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik
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(creator_code:org_t)
- 2009
- 2009
- English.
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In: Molecular Biology of the Cell. - 1059-1524 .- 1939-4586. ; 20:15, s. 3459-3470
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https://doi.org/10.1...
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Abstract
Subject headings
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- Eukaryotic cells have evolved quality control mechanisms to degrade aberrant mRNA molecules and prevent the synthesis of defective proteins that could be deleterious for the cell. The exosome, a protein complex with ribonuclease activity, is a key player in quality control. An early quality checkpoint takes place cotranscriptionally but little is known about the molecular mechanisms by which the exosome is recruited to the transcribed genes. Here we study the core exosome subunit Rrp4 in two insect model systems, Chironomus and Drosophila. We show that a significant fraction of Rrp4 is associated with the nascent pre-mRNPs and that a specific mRNA-binding protein, Hrp59/hnRNP M, interacts in vivo with multiple exosome subunits. Depletion of Hrp59 by RNA interference reduces the levels of Rrp4 at transcription sites, which suggests that Hrp59 is needed for the exosome to stably interact with nascent pre-mRNPs. Our results lead to a revised mechanistic model for cotranscriptional quality control in which the exosome is constantly recruited to newly synthesized RNAs through direct interactions with specific hnRNP proteins.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- NATURAL SCIENCES
- NATURVETENSKAP
- Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Hessle, Viktoria
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Björk, Petra
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Sokolowski, Marc ...
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Gonzalez de Vald ...
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Silverstein, Reb ...
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Artemenko, Konst ...
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show more...
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Tyagi, Anu
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Maddalo, Gianluc ...
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Ilag, Leopold
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Helbig, Roger
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Zubarev, Roman A ...
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Visa, Neus
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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Molecular Biolog ...
- By the university
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Stockholm University
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Uppsala University
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Karolinska Institutet