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Diazotrophic growth of Rhodospirillum rubrum with 2-oxoglutarate as sole carbon source affects the regulation of nitrogen metabolism as well as the soluble proteome
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- Teixeira, Pedro Filipe (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Selao, Tiago Toscano (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Henriksson, Veronika (author)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Elsevier BV, 2010
- 2010
- English.
- In: Research in Microbiology. - : Elsevier BV. - 0923-2508 .- 1769-7123. ; 161:8, s. 651-659
- Journal article (peer-reviewed)
Abstract
Subject headings
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- 2-Oxoglutarate plays a central role as a signal in the regulation of nitrogen metabolism in the phototrophic diazotroph Rhodospirillum rubrum. In order to further study the role of this metabolite, we have constructed an R. rubrum strain that has the capacity to grow on 2-oxoglutarate as sole carbon source, in contrast to wild-type R. rubrum. This strain has the same growth characteristics as wild-type with malate as carbon source, but showed clear metabolic differences when 2-oxoglutarate was used. Among other things, the regulation of nitrogen metabolism is altered, which can be related to different modification profiles of the regulatory PII proteins.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Ammonium assimilation
- Nitrogen fixation
- Regulation
- Rhodospirillum rubrum
- Biochemistry
- Biokemi
- Biochemistry
- biokemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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