Theoretical modeling of the nonenzymatic solvolysis of CMP-NeuAc in an acidic environment

• A density-functional-theory investigation of the nonenzymatic solvolysis of the cytydine 5'-monophosphate N-acetylneuraminic acid and its derivatives in the acidic environment is presented. The theoretical calculations of the second stage of the reaction mechanism are in agreement with the hypothesis of a dissociative oxocarbenium-like transition state with proton transfer as a key part of the reaction. The geometries of the transition states of the reactions yielding alpha-methyl and P-meth, I glycosides are essentially different. This study provides new theoretical data that can be helpful in elucidating the mechanism of the carbohydrates hydrolysis as well as other reactions catalyzed by the glycosyltransferases.

Subject headings

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Keyword

CMP-NeuAc

solvolysis

sialic acid

sialyltransferase

DFT

Chemistry

Kemi

Publication and Content Type

ref (subject category)

art (subject category)