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Development of a high performance anion exchange chromatography analysis for mapping of oligosaccharides
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- Grey, Carl (author)
- Lund University,Lunds universitet,Bioteknik,Centrum för tillämpade biovetenskaper,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biotechnology,Center for Applied Life Sciences,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
- Edebrink, Per (author)
- Krook, Maria (author)
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- (creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
- In: Journal of chromatography. B. - : Elsevier BV. - 1570-0232 .- 1873-376X. ; 877:20-21, s. 1827-1832
- Journal article (peer-reviewed)
Abstract
Subject headings
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- In the present study a HPAEC-PAD method is described that was developed for monitoring the consistency of N-glycosylation during the production and purification of recombinant proteins and monoclonal antibodies. The method successfully separated 18 neutral and sialylated oligosaccharides. Results obtained were compared with MALDI-TOF MS and it was shown that both methods gave similar results. in addition, a method validation was performed showing that the HPAEC-PAD analysis was well suited for the mapping and characterization of oligosaccharides. The method was found to be robust and additionally the precision was significantly better compared to the MALDI-TOF MS method.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology (hsv//eng)
Keyword
- HPAEC-PAD
- MALDI-TOF MS
- Oligosaccharides
- N-glycosylation
- Glycoprotein
- Monoclonal antibodies
- Analytical chemistry
- Analytisk kemi
- Monoclonal antibodies
- Glycoprotein
- N-glycosylation
- Oligosaccharides
- HPAEC-PAD
- MALDI-TOF MS
Publication and Content Type
- ref (subject category)
- art (subject category)
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