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A recombinant Bacil...
A recombinant Bacille Calmette-Guerin construct expressing the Plasmodium falciparum circumsporozoite protein enhances dendritic cell activation and primes for circumsporozoite-specific memory cells in BALB/c mice
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- Arama, Charles (author)
- Stockholms universitet,Avdelningen för immunologi
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- Waseem, Shahid (author)
- Stockholms universitet,Avdelningen för immunologi
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- Fernández, Carmen (author)
- Stockholms universitet,Avdelningen för immunologi
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- Assefaw-Redda, Yohannes (author)
- Stockholms universitet,Avdelningen för immunologi
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- You, Liya (author)
- Stockholms universitet,Avdelningen för immunologi
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Rodriguez, Ariane (author)
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Radošević, Katarina (author)
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Goudsmit, Jaap (author)
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Kaufmann, Stefan H E (author)
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Reece, Stephen T (author)
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- Troye-Blomberg, Marita (author)
- Stockholms universitet,Avdelningen för immunologi
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(creator_code:org_t)
- Elsevier, 2012
- 2012
- English.
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In: Vaccine. - : Elsevier. - 0264-410X .- 1873-2518. ; 30:37, s. 5578-5584
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A protective malaria vaccine may induce both high levels of neutralising antibodies and strong T-cell responses. The Plasmodium falciparum circumsporozoite protein (CSp) is a leading pre-erythrocytic vaccine candidate. CSp is a week immunogen per se, but Mycobacterium bovis Bacille Calmette-Guérin (BCG) has excellent adjuvant activity and has been utilized as a vector to deliver heterologous vaccine candidate antigens. It is safe in immunocompetent individuals and inexpensive to produce. We assessed in vitro and in vivo a recombinant BCG-expressing CSp (BCG-CS) as malaria vaccine candidate. Immunisation of BALB/c mice with BCG-CS augmented numbers of dendritic cells (DCs) in draining lymph nodes and in the spleen. The activation markers MHC-class-II, CD40, CD80 and CD86 on DCs were significantly upregulated by BCG-CS as compared to wild-type BCG (wt-BCG). In vitro stimulation of bone marrow-derived DCs and macrophages with BCG-CS induced IL-12 and TNF-α production. BCG-CS induced higher phagocytic activity in macrophages as compared to wt-BCG. Immunogenicity studies show that BCG-CS induced CS-specific antibodies and IFN-γ-producing memory cells. In conclusion, BCG-CS is highly efficient in activating antigen-presenting cells (APCs) for priming of adaptive immunity. Implications for the rational design of novel vaccines against malaria and TB, the two major devastating poverty-related diseases, are discussed.
Subject headings
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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Vaccine
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- By the author/editor
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Arama, Charles
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Waseem, Shahid
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Fernández, Carme ...
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Assefaw-Redda, Y ...
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You, Liya
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Rodriguez, Arian ...
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show more...
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Radošević, Katar ...
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Goudsmit, Jaap
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Kaufmann, Stefan ...
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Reece, Stephen T
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Troye-Blomberg, ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
- Articles in the publication
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Vaccine
- By the university
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Stockholm University