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A comparison of TO-...
A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy
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- Gorokhova, Elena (author)
- Stockholms universitet,Institutionen för tillämpad miljövetenskap (ITM),Systemekologiska institutionen
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- Mattsson, Lisa (author)
- Stockholms universitet,Systemekologiska institutionen
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- Sundström, Annica M. (author)
- Stockholms universitet,Systemekologiska institutionen
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- English.
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In: Journal of Microbiological Methods. - : Elsevier BV. - 0167-7012 .- 1872-8359. ; 89:3, s. 216-221
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Two fluorescent dyes, TO-PRO-1 iodide and 5-CFDA-AM, were evaluated for LIVE/DEAD assessment of unicellular marine algae Brachiomonas submarina and Tetraselmis suecica. Epifluorescence microscopy was used to estimate cell viability in predetermined mixtures of viable and non-viable algal cells and validated using microplate growth assay as reference measurements. On average, 5-CFDA-AM underestimated live cell abundance by similar to 25% compared with viability estimated by the growth assay, whereas TO-PRO-1 iodide provided accurate viability estimates. Furthermore, viability estimates based on staining with TO-PRO-1 iodide were not affected by a storage period of up to one month in -80 degrees C, making the assay a good candidate for routine assessment of phytoplankton populations in field and laboratory studies.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Algal cell viability
- Carboxylluorescein diacetate acetoxymethyl ester
- 5-CFDA-AM
- LIVE/DEAD staining
- Microplate growth assay
- TO-PRO-1 iodide
Publication and Content Type
- ref (subject category)
- art (subject category)
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