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Preferential Genome...
Preferential Genome Targeting of the CBP Co-Activator by Rel and Smad Proteins in Early Drosophila melanogaster Embryos
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- Holmqvist, Per-Henrik (author)
- Stockholms universitet,Wenner-Grens institut,The Wenner-Gren Institute, Developmental Biology, Stockholm University, Stockholm
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- Boija, Ann (author)
- Stockholms universitet,Wenner-Grens institut,The Wenner-Gren Institute, Developmental Biology, Stockholm University, Stockholm
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- Philip, Philge (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Per Stenberg, Computational Life Science Cluster (CLiC)
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- Crona, Filip, 1977- (author)
- Stockholms universitet,Wenner-Grens institut,The Wenner-Gren Institute, Developmental Biology, Stockholm University, Stockholm
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- Stenberg, Per, 1974- (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Computational Life Science Cluster (CLiC)
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- Mannervik, Mattias (author)
- Stockholms universitet,Wenner-Grens institut,The Wenner-Gren Institute, Developmental Biology, Stockholm University, Stockholm
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(creator_code:org_t)
- 2012-06-21
- 2012
- English.
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In: PLOS Genetics. - San Francisco : Public Library of Science (PLoS). - 1553-7390 .- 1553-7404. ; 8:6
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Abstract
Subject headings
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- CBP and the related p300 protein are widely used transcriptional co-activators in metazoans that interact with multiple transcription factors. Whether CBP/p300 occupies the genome equally with all factors or preferentially binds together with some factors is not known. We therefore compared Drosophila melanogaster CBP (nejire) ChIP-seq peaks with regions bound by 40 different transcription factors in early embryos, and we found high co-occupancy with the Rel-family protein Dorsal. Dorsal is required for CBP occupancy in the embryo, but only at regions where few other factors are present. CBP peaks in mutant embryos lacking nuclear Dorsal are best correlated with TGF-beta/Dpp-signaling and Smad-protein binding. Differences in CBP occupancy in mutant embryos reflect gene expression changes genome-wide, but CBP also occupies some non-expressed genes. The presence of CBP at silent genes does not result in histone acetylation. We find that Polycomb-repressed H3K27me3 chromatin does not preclude CBP binding, but restricts histone acetylation at CBP-bound genomic sites. We conclude that CBP occupancy in Drosophila embryos preferentially overlaps factors controlling dorsoventral patterning and that CBP binds silent genes without causing histone hyperacetylation.
Subject headings
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Keyword
- utvecklingsbiologi
- Developmental Biology
- genetik
Publication and Content Type
- ref (subject category)
- art (subject category)
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