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Room temperature fe...
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals
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Kern, Jan (author)
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Alonso-Mori, Roberto (author)
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Hellmich, Julia (author)
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Tran, Rosalie (author)
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Hattne, Johan (author)
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Laksmono, Hartawan (author)
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Gloeckner, Carina (author)
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Echols, Nathaniel (author)
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Sierra, Raymond G. (author)
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- Sellberg, Jonas (author)
- Stockholms universitet,Fysikum
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Lassalle-Kaiser, Benedikt (author)
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Gildea, Richard J. (author)
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Glatzel, Pieter (author)
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Grosse-Kunstleve, Ralf W. (author)
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Latimer, Matthew J. (author)
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McQueen, Trevor A. (author)
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DiFiore, Doerte (author)
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Fry, Alan R. (author)
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- Seibert, M Marvin (author)
- Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, CA 94025
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Miahnahri, Alan (author)
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Schafer, Donald W. (author)
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Seibert, M. Marvin (author)
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Sokaras, Dimosthenis (author)
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Weng, Tsu-Chien (author)
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Zwart, Petrus H. (author)
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White, William E. (author)
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Adams, Paul D. (author)
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Bogan, Michael J. (author)
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Boutet, Sebastien (author)
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Williams, Garth J. (author)
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- Messinger, Johannes (author)
- Umeå universitet,Kemiska institutionen
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Sauter, Nicholas K. (author)
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Zouni, Athina (author)
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Bergmann, Uwe (author)
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Yano, Junko (author)
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Yachandra, Vittal K. (author)
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(creator_code:org_t)
- 2012-06-04
- 2012
- English.
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 109:25, s. 9721-9726
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https://doi.org/10.1...
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https://www.pnas.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This lightdriven, four-photon reaction is catalyzed by the Mn4CaO5 cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However, the necessity for collecting data at room temperature, especially for studying the transient steps during the O-O bond formation, requires the development of new methodologies. In this paper we report room temperature X-ray diffraction data of PS II microcrystals obtained using ultrashort (<50 fs) 9 keV X-ray pulses from a hard X-ray free electron laser, namely the Linac Coherent Light Source. The results presented here demonstrate that the probe before destroy approach using an X-ray free electron laser works even for the highly-sensitive Mn4CaO5 cluster in PS II at room temperature. We show that these data are comparable to those obtained in synchrotron radiation studies as seen by the similarities in the overall structure of the helices, the protein subunits and the location of the various cofactors. This work is, therefore, an important step toward future studies for resolving the structure of the Mn4CaO5 cluster without any damage at room temperature, and of the reaction intermediates of PS II during O-O bond formation.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
Keyword
- manganese
- oxygen-evolving complex
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Kern, Jan
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Alonso-Mori, Rob ...
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Hellmich, Julia
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Tran, Rosalie
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Hattne, Johan
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Laksmono, Hartaw ...
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show more...
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Gloeckner, Carin ...
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Echols, Nathanie ...
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Sierra, Raymond ...
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Sellberg, Jonas
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Lassalle-Kaiser, ...
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Gildea, Richard ...
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Glatzel, Pieter
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Grosse-Kunstleve ...
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Latimer, Matthew ...
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McQueen, Trevor ...
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DiFiore, Doerte
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Fry, Alan R.
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Seibert, M Marvi ...
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Miahnahri, Alan
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Schafer, Donald ...
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Seibert, M. Marv ...
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Sokaras, Dimosth ...
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Weng, Tsu-Chien
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Zwart, Petrus H.
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White, William E ...
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Adams, Paul D.
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Bogan, Michael J ...
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Boutet, Sebastie ...
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Williams, Garth ...
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Messinger, Johan ...
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Sauter, Nicholas ...
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Zouni, Athina
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Bergmann, Uwe
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Yano, Junko
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Yachandra, Vitta ...
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show less...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
- Articles in the publication
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Proceedings of t ...
- By the university
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Stockholm University
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Umeå University
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Uppsala University