Simultaneous Femtosecond X-ray Spectroscopy and Diffraction of Photosystem II at Room Temperature

AuthorCount:41;
Intense femtosecond x-ray pulses produced at the Linac Coherent Light Source (LCLS) were used for simultaneous x-ray diffraction (XRD) and x-ray emission spectroscopy (XES) of microcrystals of photosystem II (PS II) at room temperature. This method probes the overall protein structure and the electronic structure of the Mn4CaO5 cluster in the oxygen-evolving complex of PS II. XRD data are presented from both the dark state (S-1) and the first illuminated state (S-2) of PS II. Our simultaneous XRD-XES study shows that the PS II crystals are intact during our measurements at the LCLS, not only with respect to the structure of PS II, but also with regard to the electronic structure of the highly radiation-sensitive Mn4CaO5 cluster, opening new directions for future dynamics studies.

Alonso-Mori, Roberto (author)
Tran, Rosalie (author)
Hattne, Johan (author)
Gildea, Richard J. (author)
Echols, Nathaniel (author)
Gloeckner, Carina (author)
Hellmich, Julia (author)
Laksmono, Hartawan (author)
Sierra, Raymond G. (author)
Lassalle-Kaiser, Benedikt (author)
Koroidov, SergeyUmeå universitet,Kemiska institutionen(Swepub:umu)seko0001 (author)
Lampe, Alyssa (author)
Han, Guangye (author)
Gul, Sheraz (author)
DiFiore, Doerte (author)
Milathianaki, Despina (author)
Fry, Alan R. (author)
Miahnahri, Alan (author)
Schafer, Donald W. (author)
Seibert, M MarvinLinac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA.(Swepub:uu)masei255 (author)
Seibert, M. Marvin (author)
Koglin, Jason E. (author)
Sokaras, Dimosthenis (author)
Weng, Tsu-Chien (author)
Sellberg, JonasStockholms universitet,Fysikum(Swepub:su)jose4950 (author)
Latimer, Matthew J. (author)
Grosse-Kunstleve, Ralf W. (author)
Zwart, Petrus H. (author)
White, William E. (author)
Glatzel, Pieter (author)
Adams, Paul D. (author)
Bogan, Michael J. (author)
Williams, Garth J. (author)
Boutet, Sebastien (author)
Messinger, JohannesUmeå universitet,Kemiska institutionen(Swepub:umu)jome0007 (author)
Zouni, Athina (author)
Sauter, Nicholas K. (author)
Yachandra, Vittal K. (author)
Bergmann, Uwe (author)
Yano, Junko (author)
Umeå universitetKemiska institutionen (creator_code:org_t)

In:Science: American Association for the Advancement of Science (AAAS)340:6131, s. 491-4950036-80751095-9203

By the author/editor: Kern, Jan; Alonso-Mori, Rob ...; Tran, Rosalie; Hattne, Johan; Gildea, Richard ...; Echols, Nathanie ...; show more...; Gloeckner, Carin ...; Hellmich, Julia; Laksmono, Hartaw ...; Sierra, Raymond ...; Lassalle-Kaiser, ...; Koroidov, Sergey; Lampe, Alyssa; Han, Guangye; Gul, Sheraz; DiFiore, Doerte; Milathianaki, De ...; Fry, Alan R.; Miahnahri, Alan; Schafer, Donald ...; Seibert, M Marvi ...; Seibert, M. Marv ...; Koglin, Jason E.; Sokaras, Dimosth ...; Weng, Tsu-Chien; Sellberg, Jonas; Latimer, Matthew ...; Grosse-Kunstleve ...; Zwart, Petrus H.; White, William E ...; Glatzel, Pieter; Adams, Paul D.; Bogan, Michael J ...; Williams, Garth ...; Boutet, Sebastie ...; Messinger, Johan ...; Zouni, Athina; Sauter, Nicholas ...; Yachandra, Vitta ...; Bergmann, Uwe; Yano, Junko; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

By the university: Stockholm University; Umeå University; Uppsala University

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