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CMV Seropositive Ch...
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Sohlberg, Ebba,1982-Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
(author)
CMV Seropositive Children Show Inhibition of In Vitro EBV Infection that is Associated with CD8+CD57+ T-cell Enrichment and IFN-g
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LIBRIS-ID:oai:DiVA.org:su-93008
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https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-93008URI
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Language:English
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Summary in:English
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Subject category:vet swepub-contenttype
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Subject category:ovr swepub-publicationtype
Notes
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Epstein-Barr virus (EBV), a human herpesvirus, is commonly acquired during childhood and persists latently in B cells. EBV seropositivity has been connected to immune modulatory effects such as altered T- and NK-cell functional responses as well as protection against early IgE-sensitization, but due to the asymptomatic presentation during childhood little is known regarding the infection process in children of different ages. Here, we used mononuclear cells from cord blood, 2-year and 5-year old EBV-naïve children for in vitro EBV infection. We show that the degree of EBV-induced B-cell activation and expansion differs between age groups and in particular in relation to IFN-g production capacity. EBV infection induced redistribution between B-cell subsets with enrichment of IgD+CD27+ cells (commonly referred to as non-switched memory) in infected cord blood cell cultures, and of IgD-CD27+ cells (switched memory) in cell cultures of older children. We also related results to serostatus to cytomegalovirus (CMV), a persistent herpesvirus that can affect differentiation status of T- and NK cells. As compared to CMV- children, the EBV-induced enrichment of IgD-CD27+ B cells was significantly reduced in infected cell cultures from CMV+ children. This effect was associated with high levels of IFN-g and frequencies of highly mature CD8+CD57+ T cells in CMV+ children. Our results demonstrate that both a child’s age and serostatus to CMV will have an impact on EBV-induced B-cell activation and expansion, and points to the ability of viruses with immune-modulatory functions, like CMV, to impact on immune responses within the host system.
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Saghafian-Hedengren, ShanieStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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Rasul, Eashan
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Marchini, Giovanna
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Nilsson, Caroline
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Klein, Eva
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Nagy, Noémi
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Sverremark-Ekström, EvaStockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut(Swepub:su)esver
(author)
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Stockholms universitetInstitutionen för molekylär biovetenskap, Wenner-Grens institut
(creator_code:org_t)
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