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Molecularly imprint...
Molecularly imprinted polymer in microextraction by packed sorbent for the simultaneous determination of local anesthetics : lidocaine, ropivacaine, mepivacaine and bupivacaine in plasma and urine samples
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- Daryanavard, Seyed (author)
- Stockholms universitet,Institutionen för analytisk kemi
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- Jeppsson-Dadoun, Amin (author)
- Stockholms universitet,Institutionen för analytisk kemi
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Andersson, Lars I. (author)
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Hashemi, Mahdi (author)
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- Colmsjö, Anders (author)
- Stockholms universitet,Institutionen för analytisk kemi
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- Abdel-Rehim, Mohamed (author)
- Stockholms universitet,Institutionen för analytisk kemi
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(creator_code:org_t)
- 2013-07-05
- 2013
- English.
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In: BMC Biomedical chromotography. - : Wiley-Blackwell. - 0269-3879 .- 1099-0801. ; 27:11, s. 1418-1488
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- This study presents the use of molecularly imprinted polymer (MIP) as packing material for microextraction by packed syringe (MEPS) to achieve higher extraction selectivity. Pentycaine was used as template for MIP. Development and validation of the determination of lidocaine, ropivacaine, mepivacaine and bupivacaine in human plasma and urine samples utilizing MIP-MEPS and liquid chromatography–tandem mass spectrometry (LC-MS/MS) were carried out. The MEPS MIP-cartridge could be used for 100 extractions before it was discarded. The extraction recovery ranged from 60 to 80%. The correlation coefficients values were >0.999 for all assays using lidocaine, ropivacaine, mepivacaine and bupivacaine in the calibration range 5–2000 nmol/L. The accuracy of the studied compounds, given as a percentage variation from the nominal concentration values, ranged from -4.9 to 8.4% using plasma and urine samples. The between-batch precision, given as the relative standard deviation, at three different concentrations (quality control samples) was ranged from −4.7 to 14.0% and from 1.8 to 12.7% in plasma and urine, respectively. The lower limit of quantification and limit of detection of the studied substances were 5.0 and 1.0 nm, respectively
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- MIP
- MEPS
- plasma and urine samples
- local anesthetic drugs
- LC-MS/MS
Publication and Content Type
- ref (subject category)
- art (subject category)
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