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Enzyme-Linked Immun...
Enzyme-Linked Immunosorbent Assay for Screening Dioxin Soil Contamination by Uncontrolled Combustion During Informal Recycling in Slums
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- Trindade, Mirta (author)
- Catedra de Bioquímica, Faculty of Chemistry, University de la República, Montevideo, Uruguay
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- Nording, Malin (author)
- Umeå universitet,Kemiska institutionen,Department of Entomology and Cancer Research Center, University of California, Davis, California 95616, USA
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- Nichkova, Mikaela Ivanova (author)
- Department of Entomology and Cancer Research Center, University of California, Davis, California 95616, USA
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- Spinnel, Erik (author)
- Umeå universitet,Kemiska institutionen
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- Haglund, Peter (author)
- Umeå universitet,Kemiska institutionen
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- Last, Michael S (author)
- Department of Pulmonary and Critical Care Medicine, University of California, Davis, California 95616, USA
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- Gee, Shirley (author)
- Department of Entomology and Cancer Research Center, University of California, Davis, California 95616, USA
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- Hammock, Bruce (author)
- Department of Entomology and Cancer Research Center, University of California, Davis, California 95616, USA
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- Last, Jerold A (author)
- Department of Pulmonary and Critical Care Medicine, University of California, Davis, California 95616, USA
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- González-Sapienza, Gualberto (author)
- Catedra de Inmunología, Department of Biosciences, Faculty of Chemistry, University de la República, Montevideo, Uruguay
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- Brena, Beatriz M (author)
- Catedra de Bioquímica, Faculty of Chemistry, University de la República, Montevideo, Uruguay and Laboratory of Environmental Quality, Intendencia Municipal de Montevideo, Montevideo, Uruguay
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(creator_code:org_t)
- John Wiley & Sons, 2008
- 2008
- English.
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In: Environmental Toxicology and Chemistry. - : John Wiley & Sons. - 0730-7268 .- 1552-8618. ; 27:11, s. 2224-2232
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Uncontrolled combustion due to garbage recycling is a widespread activity among slum dwellers in distressed economy countries, and has been indicated as a major source of dioxin contamination. However, due to the high cost and complexity of gas chromatography-high resolution mass spectrometry (GC-HRMS) analysis, the magnitude of the problem remains largely unknown. This work describes a first approach towards the use of a dioxin antibody-based enzyme-linked immunosorbent assay (ELISA) as the basis for a sustainable, simple, and low cost monitoring program to assess the toxicological impact of uncontrolled combustion in slums. A panel of sixteen samples was analyzed by GC-HRMS and ELISA on split extracts. Close to 20 % of the analyzed samples showed dioxin concentrations up to almost twice the guidance level for residential soil in several countries, pointing out the need for performing a large-scale monitoring program. Despite the potential for variations in dioxin congener distribution due to the mixed nature of the incinerated material, there was a good correlation between the toxic equivalents as determined by GC-HRMS and ELISA. Furthermore, an inter-laboratory ELISA validation showed that the capacity to perform the dioxin ELISA was successfully transferred between laboratories. It was concluded that the ELISA method performed very well as a screening tool to prioritize samples for instrumental analysis, which allows cutting down costs significantly.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Polychlorinated dibenzo-p-dioxins/furans
- immunoassays
- dioxin
- slum contamination
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Trindade, Mirta
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Nording, Malin
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Nichkova, Mikael ...
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Spinnel, Erik
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Haglund, Peter
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Last, Michael S
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show more...
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Gee, Shirley
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Hammock, Bruce
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Last, Jerold A
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González-Sapienz ...
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Brena, Beatriz M
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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Environmental To ...
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Umeå University