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Detection and Isola...
Detection and Isolation of Sindbis Virus from Mosquitoes Captured During an Outbreak in Sweden, 2013
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- Bergqvist, Joakim (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Forsman, Oscar (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Larsson, Pär (author)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Näslund, Jonas (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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Lilja, Tobias (author)
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- Engdahl, Cecilia (author)
- Umeå universitet,Kemiska institutionen
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Lindström, Anders (author)
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- Gylfe, Åsa (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Ahlm, Clas (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Arcum
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- Evander, Magnus (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Arktiskt centrum vid Umeå universitet (Arcum),Arcum
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- Bucht, Göran (author)
- [ 1 ] CBRN Def & Secur, Swedish Def Res Agcy, SE-90182 Umea, Sweden
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(creator_code:org_t)
- Mary Ann Liebert Inc, 2015
- 2015
- English.
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In: Vector Borne and Zoonotic Diseases. - : Mary Ann Liebert Inc. - 1530-3667 .- 1557-7759. ; 15:2, s. 133-140
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https://umu.diva-por... (primary) (Raw object)
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http://umu.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Mosquito-borne alphaviruses have the potential to cause large outbreaks throughout the world. Here we investigated the causative agent of an unexpected Sindbis virus (SINV) outbreak during August-September, 2013, in a previously nonendemic region of Sweden. Mosquitoes were collected using carbon dioxide-baited CDC traps at locations close to human cases. The mosquitoes were initially screened as large pools by SINV-specific quantitative RT-PCR, and the SINV-positive mosquitoes were species determined by single-nucleotide polymorphism (SNP) analysis, followed by sequencing the barcoding region of the cytochrome oxidase I gene. The proportion of the collected mosquitoes was determined by a metabarcoding strategy. By using novel strategies for PCR screening and genetic typing, a new SINV strain, Lovanger, was isolated from a pool of 1600 mosquitoes composed of Culex, Culiseta, and Aedes mosquitoes as determined by metabarcoding. The SINV-positive mosquito Culiseta morsitans was identified by SNP analysis and sequencing. After whole-genome sequencing and phylogenetic analysis, the SINV Lovanger isolate was shown to be most closely similar to recent Finnish SINV isolates. In conclusion, within a few weeks, we were able to detect and isolate a novel SINV strain and identify the mosquito vector during a sudden SINV outbreak.
Keyword
- Barcoding
- Mosquito
- Isolation
- Virus
- Sindbis
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Bergqvist, Joaki ...
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Forsman, Oscar
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Larsson, Pär
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Näslund, Jonas
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Lilja, Tobias
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Engdahl, Cecilia
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show more...
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Lindström, Ander ...
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Gylfe, Åsa
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Ahlm, Clas
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Evander, Magnus
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Bucht, Göran
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- Articles in the publication
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Vector Borne and ...
- By the university
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Umeå University