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Online solid phase ...
Online solid phase extraction liquid chromatography using bonded zwitterionic stationary phases and tandem mass spectrometry for rapid environmental trace analysis of highly polar hydrophilic compounds – Application for the antiviral drug Zanamivir
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- Lindberg, Richard H. (author)
- Umeå universitet,Kemiska institutionen
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- Fedorova, Ganna (author)
- Umeå universitet,Kemiska institutionen,University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech Republic
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- Blum, Kristin M. (author)
- Umeå universitet,Kemiska institutionen
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Pulit-Prociak, Jolanta (author)
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- Gillman, Anna (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Klinisk mikrobiologi och infektionsmedicin,Infektionssjukdomar
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- Järhult, Josef (author)
- Uppsala universitet,Klinisk mikrobiologi och infektionsmedicin,Institutionen för medicinsk biokemi och mikrobiologi,Infektionssjukdomar
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Appelblad, Patrik (author)
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- Söderström, Hanna (author)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- Elsevier, 2015
- 2015
- English.
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In: Talanta. - : Elsevier. - 0039-9140 .- 1873-3573. ; 141, s. 164-169
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Abstract
Subject headings
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- Abstract Zanamivir (Za) is a highly polar and hydrophilic antiviral drug used for the treatment of influenza A viruses. Za has been detected in rivers of Japan and it's environmental occurrence has the risk of inducing antiviral resistant avian influenza viruses. In this study, a rapid automated online solid phase extraction liquid chromatography method using bonded zwitterionic stationary phases and tandem mass spectrometry (SPE/LC–MS/MS) for trace analysis of Za was developed. Furthermore, an internal standard (IS) calibration method capable of quantifying Za in Milli-Q, surface water, sewage effluent and sewage influent was evaluated. Optimum pre-extraction sample composition was found to be 95/5 v/v acetonitrile/water sample and 1% formic acid. The developed method showed acceptable linearities (r2≥0.994), filtration recovery (≥91%), and intra-day precisions (RSD≤16%), and acceptable and environmentally relevant LOQs (≤20 ng L−1). Storage tests showed no significant losses of Za during 20 days and +4/−20 °C (≤12%) with the exception of influent samples, which should be kept at −20 °C to avoid significant Za losses. The applicability of the method was demonstrated in a study on phototransformation of Za in unfiltered and filtered surface water during 28 days of artificial UV irradiation exposure. No significant (≤12%) phototransformation was found in surface water after 28 days suggesting a relatively high photostability of Za and that Za should be of environmental concern.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Antivirals
- Zanamivir
- Online solid phase extraction
- Liquid chromatography
- ZIC-HILIC
- Tandem mass spectrometry
Publication and Content Type
- ref (subject category)
- art (subject category)
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