Search: onr:"swepub:oai:DiVA.org:umu-106595" >
Scaffolding of a ba...
Scaffolding of a bacterial genome using MinION nanopore sequencing
- Article/chapterEnglish2015
Publisher, publication year, extent ...
-
2015-07-07
-
Springer Science and Business Media LLC,2015
-
electronicrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:umu-106595
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-106595URI
-
https://doi.org/10.1038/srep11996DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Second generation sequencing has revolutionized genomic studies. However, most genomes contain repeated DNA elements that are longer than the read lengths achievable with typical sequencers, so the genomic order of several generated contigs cannot be easily resolved. A new generation of sequencers offering substantially longer reads is emerging, notably the Pacific Biosciences (PacBio) RS II system and the MinION system, released in early 2014 by Oxford Nanopore Technologies through an early access program. The latter has highly advantageous portability and sequences samples by measuring changes in ionic current when single-stranded DNA molecules are translocated through nanopores. We show that the MinION system produces long reads with high mapability that can be used for scaffolding bacterial genomes, despite currently producing substantially higher error rates than PacBio reads. With further development we anticipate that MinION will be useful not only for assembling genomes, but also for rapid detection of organisms, potentially in the field.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Larkeryd, A.
(author)
-
Sjödin, AndreasUmeå universitet,Kemiska institutionen,Swedish Defence Research Agency, Umeå, Sweden(Swepub:umu)anssjn96
(author)
-
Forsman, M.
(author)
-
Stenberg, PerUmeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Swedish Defence Research Agency, Umeå, Sweden; Department of Chemistry, Computational Life Science Cluster (CLiC), Umeå University, Umeå, Sweden(Swepub:umu)perstg96
(author)
-
Umeå universitetKemiska institutionen
(creator_code:org_t)
Related titles
-
In:Scientific Reports: Springer Science and Business Media LLC52045-2322
Internet link
Find in a library
To the university's database