Localization of silencer and enhancer elements in the human type X collagen gene.

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Localization of silencer and enhancer elements in the human type X collagen gene.

Beier, Frank (author): Institut für Experimentelle Medizin, Universität Erlangen-Nürnberg, Erlangen, Germany; Department of Medical Biochemistry, University of Calgary, Calgary, Canada

Vornehm, Silvia (author): Institut für Experimentelle Medizin, Universität Erlangen-Nürnberg, Erlangen, Germany

Pöschl, Ernst (author): Institut für Experimentelle Medizin, Universität Erlangen-Nürnberg, Erlangen, Germany

von der Mark, Klaus (author): Institut für Experimentelle Medizin, Universität Erlangen-Nürnberg, Erlangen, Germany

Lammi, Mikko, 1961- (author): Department of Anatomy, University of Kuopio, Kuopio, Finland,orcid.org/0000-0002-6181-9904

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John Wiley & Sons, 1997
1997
English.
In: Journal of Cellular Biochemistry. - : John Wiley & Sons. - 0730-2312 .- 1097-4644. ; 66:2, s. 210-218

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Journal article (peer-reviewed)

Abstract Subject headings

Collagen type X is a short, network-forming collagen expressed temporally and spatially tightly controlled in hypertrophic chondrocytes during endochondral ossification. Studies on chicken chondrocytes indicate that the regulation of type X collagen gene expression is regulated at the transcriptional level. In this study, we have analyzed the regulatory elements of the human type X collagen (Col10a1) by reporter gene constructs and transient transfections in chondrogenic and nonchondrogenic cells. Four different promoter fragments covering up to 2,864 bp of 5'-flanking sequences, either including or lacking the first intron, were linked to luciferase reporter gene and transfected into 3T3 fibroblasts, HT1080 fibrosarcoma cells, prehypertrophic chondrocytes from the resting zone, hypertrophic chondrocytes, and chondrogenic cell lines. The results indicated the presence of three regulatory elements in the human Col10a1 gene besides the proximal promoter. First, a negative regulatory element located between 2.4 and 2.8 kb upstream of the transcription initiation site was active in all nonchondrogenic cells and in prehypertrophic chondrocytes. Second, a positive, but also non-tissue-specific positive regulatory element was present in the first intron. Third, a cell-type-specific enhancer element active only in hypertrophic chondrocytes was located between -2.4 and -0.9 kb confirming a previous report by Thomas et al. [(1995): Gene 160:291-296]. The enhancing effect, however, was observed only when calcium phosphate was either used for transfection or included in the culture medium after lipofection. These findings demonstrate that the rigid control of human Col10a1 gene expression is achieved by both positive and negative regulatory elements in the gene and provide the basis for the identification of factors binding to those elements.

Subject headings

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)

Keyword

Gene regulation
chondrocyte
type X collagen
response elements

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By the author/editor: Beier, Frank; Vornehm, Silvia; Pöschl, Ernst; von der Mark, Kl ...; Lammi, Mikko, 19 ...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Cell Biology

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Genetics

Articles in the publication: Journal of Cellu ...

By the university: Umeå University

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