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Myocardial ischemic...
Myocardial ischemic preconditioning in a porcine model leads to rapid changes in cardiac extracellular vesicle messenger RNA content
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- Svennerholm, Kristina, 1981 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för anestesiologi och intensivvård,Institute of Clinical Sciences, Department of Anesthesiology and Intensive care
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- Rodsand, Pouria (författare)
- Umeå universitet,Anestesiologi och intensivvård,Umeå Univ, Dept Surg & Perioperat Sci, Anesthesiol & Intens Care Med, S-90185 Umeå, Sweden.
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- Hellman, Urban, 1966- (författare)
- Umeå universitet,Kardiologi,Umeå Univ, Dept Publ Hlth & Clin Med, Cardiol, S-90185 Umeå, Sweden.;Umeå Univ, Ctr Heart, Cardiol, S-90185 Umeå, Sweden.
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- Lundholm, Marie (författare)
- Umeå universitet,Patologi,Umeå Univ, Dept Med Biosci, Pathol, S-90185 Umeå, Sweden.
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- Waldenström, Anders (författare)
- Umeå universitet,Kardiologi,Umeå Univ, Dept Publ Hlth & Clin Med, Cardiol, S-90185 Umeå, Sweden.;Umeå Univ, Ctr Heart, Cardiol, S-90185 Umeå, Sweden.
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- Biber, Björn, 1944 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för anestesiologi och intensivvård,Institute of Clinical Sciences, Department of Anesthesiology and Intensive care
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- Ronquist, Gunnar (författare)
- Uppsala universitet,Klinisk kemi
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- Haney, Michael (författare)
- Umeå universitet,Anestesiologi och intensivvård,Umeå Univ, Dept Surg & Perioperat Sci, Anesthesiol & Intens Care Med, S-90185 Umeå, Sweden.
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(creator_code:org_t)
- Elsevier, 2015
- 2015
- Engelska.
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Ingår i: International Journal of Cardiology Heart and Vasculature. - : Elsevier. - 2352-9067. ; 8, s. 62-67
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- Background: Extracellular vesicles (EVs) are thought to exert protective effects after ischemic and remote ischemic preconditioning. It is not well understood which EV content factors are most relevant for protective effects. We hypothesize that ischemic preconditioning leads to qualitative changes in EV mRNA content and quantitative changes in EV size and number.Methods: Using an in vivo porcine ischemic preconditioning model, EVs were collected from coronary venous blood, and isolated by differential ultracentrifugations. The presence and purity of EV were verified by electron microscopy and Western blot, and EV number was assessed by nanoparticle tracking analysis. The mRNA EV was identified by microarray.Results: Gene ontology analysis showed enrichment of EV mRNA coding for proteins associated with regulation of transcription, translation, extracellular matrix, morphogenic development and feeding behavior. There were 11,678 different mRNA transcripts detected in EV, where a total of 1103 was significantly increased or decreased after preconditioning, of which 638 mRNA sequences were up-regulated and/or emerged due to preconditioning. Several of them have known association with ischemic preconditioning. There was no significant difference in EV quantity or size before and after preconditioning.Conclusions: These findings demonstrate in an in vivo model that myocardial ischemic preconditioning influences the composition of mRNA in EV, including gene transcripts for proteins associated with the protective effect of ischemic preconditioning. The finding that preconditioned parental cells release EV containing mRNA that is qualitatively different from those released by non-preconditioned cells shows the importance of the external milieu on parental cell EV production.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)
Nyckelord
- extracellular vesicles
- mRNA
- transcription
- ischemic preconditioning
- myocardium
- kardiologi
- Cardiology
- Extracellular vesicles
- Ischemic preconditioning
- MRNA
- Myocardium
- Transcription
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Svennerholm, Kri ...
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Rodsand, Pouria
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Hellman, Urban, ...
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Lundholm, Marie
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Waldenström, And ...
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Biber, Björn, 19 ...
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Ronquist, Gunnar
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Haney, Michael
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