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Identification of m...
Identification of molecular signatures specific for distinct cranial sensory ganglia in the developing chick
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- Patthey, Cedric (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Department of Zoology, University of Oxford, Oxford, UK
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Clifford, Harry (author)
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Haerty, Wilfried (author)
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Ponting, Chris P. (author)
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Shimeld, Sebastian M. (author)
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Begbie, Jo (author)
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(creator_code:org_t)
- 2016-01-27
- 2016
- English.
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In: Neural Development. - : Springer Science and Business Media LLC. - 1749-8104. ; 11
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Abstract
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- Background: The cranial sensory ganglia represent populations of neurons with distinct functions, or sensory modalities. The production of individual ganglia from distinct neurogenic placodes with different developmental pathways provides a powerful model to investigate the acquisition of specific sensory modalities. To date there is a limited range of gene markers available to examine the molecular pathways underlying this process.Results: Transcriptional profiles were generated for populations of differentiated neurons purified from distinct cranial sensory ganglia using microdissection in embryonic chicken followed by FAC-sorting and RNAseq. Whole transcriptome analysis confirmed the division into somato- versus viscerosensory neurons, with additional evidence for subdivision of the somatic class into general and special somatosensory neurons. Cross-comparison of distinct ganglia transcriptomes identified a total of 134 markers, 113 of which are novel, which can be used to distinguish trigeminal, vestibulo-acoustic and epibranchial neuronal populations. In situ hybridisation analysis provided validation for 20/26 tested markers, and showed related expression in the target region of the hindbrain in many cases.Conclusions: One hundred thirty-four high-confidence markers have been identified for placode-derived cranial sensory ganglia which can now be used to address the acquisition of specific cranial sensory modalities.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Keyword
- Cranial sensory ganglia
- Viscerosensory neuron
- Somatosensory neuron
- Cell type markers
- Chicken
Publication and Content Type
- ref (subject category)
- art (subject category)
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