Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins.

Svec, Frantisek (author)

Fréchet, Jean M. J. (author)

(creator_code:org_t)

Wiley, 2006
2006
English.
In: Journal of Separation Science. - : Wiley. - 1615-9306 .- 1615-9314. ; 29:1, s. 25-32

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; show less...

Abstract Subject headings

Monolithic capillary columns for hydrophobic interaction chromatography (HIC) have been prepared by thermally initiated, single-step in situ polymerization of mixtures of monovinyl monomers including butyl methacrylate and/or 2-hydroxyethyl methacrylate, with a divinyl crosslinker glycerol dimethacrylate or 1,4-butanediol dimethacrylate using two different porogen systems. Two porogenic solvent mixtures were used; one "hydrophilic", consisting of water, butanediol, and propanol, and one "hydrophobic," comprising dodecanol and cyclohexanol. The porous structures of the monoliths were characterized and their performance was demonstrated with a separation of a mixture of myoglobin, ribonuclease A, and lysozyme under conditions typical of HIC.

By the author/editor: Hemström, Petrus; Nordborg, Anna; Irgum, Knut; Svec, Frantisek; Fréchet, Jean M. ...

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