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Polymer-based monol...
Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins.
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- Hemström, Petrus (author)
- Umeå universitet,Kemiska institutionen
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- Nordborg, Anna (author)
- Umeå universitet,Kemiska institutionen
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- Irgum, Knut (author)
- Umeå universitet,Kemiska institutionen
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Svec, Frantisek (author)
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Fréchet, Jean M. J. (author)
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(creator_code:org_t)
- Wiley, 2006
- 2006
- English.
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In: Journal of Separation Science. - : Wiley. - 1615-9306 .- 1615-9314. ; 29:1, s. 25-32
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Monolithic capillary columns for hydrophobic interaction chromatography (HIC) have been prepared by thermally initiated, single-step in situ polymerization of mixtures of monovinyl monomers including butyl methacrylate and/or 2-hydroxyethyl methacrylate, with a divinyl crosslinker glycerol dimethacrylate or 1,4-butanediol dimethacrylate using two different porogen systems. Two porogenic solvent mixtures were used; one "hydrophilic", consisting of water, butanediol, and propanol, and one "hydrophobic," comprising dodecanol and cyclohexanol. The porous structures of the monoliths were characterized and their performance was demonstrated with a separation of a mixture of myoglobin, ribonuclease A, and lysozyme under conditions typical of HIC.
Keyword
- Capillary
- Column
- HIC
- Hydrophobic interaction chromatography
- Monolith
- Protein separation
Publication and Content Type
- ref (subject category)
- art (subject category)
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