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Intra-islet lesions...
Intra-islet lesions and lobular variations in β-cell mass expansion in ob/ob mice revealed by 3D imaging of intact pancreas
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- Parween, Saba (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM),Ulf Ahlgren
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- Kostromina, Elena (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Nord, Christoffer (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Eriksson, Maria (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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- Lindström, Per (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Ahlgren, Ulf (author)
- Umeå universitet,Umeå centrum för molekylär medicin (UCMM)
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(creator_code:org_t)
- 2016-10-07
- 2016
- English.
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In: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 6
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Abstract
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- The leptin deficient ob/ob mouse is a widely used model for studies on initial aspects of metabolic disturbances leading to type 2 diabetes, including insulin resistance and obesity. Although it is generally accepted that ob/ob mice display a dramatic increase in β-cell mass to compensate for increased insulin demand, the spatial and quantitative dynamics of β-cell mass distribution in this model has not been assessed by modern optical 3D imaging techniques. We applied optical projection tomography and ultramicroscopy imaging to extract information about individual islet β-cell volumes throughout the volume of ob/ob pancreas between 4 and 52 weeks of age. Our data show that cystic lesions constitute a significant volume of the hyperplastic ob/ob islets. We propose that these lesions are formed by a mechanism involving extravasation of red blood cells/plasma due to increased islet vessel blood flow and vessel instability. Further, our data indicate that the primary lobular compartments of the ob/ob pancreas have different potentials for expanding their β-cell population. Unawareness of the characteristics of β-cell expansion in ob/ob mice presented in this report may significantly influence ex vivo and in vivo assessments of this model in studies of β-cell adaptation and function.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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