The chloroplast lumen and stromal proteomes of Arabidopsis thaliana show differential sensitivity to short- and long-term exposure to low temperature.

Goulas, EstelleUmeå universitet / Université des Sciences et Technologies de Lille 1, France (author)

Som manuskript i avhandling. As manuscript in dissertation.
Cold acclimation and over-wintering by herbaceous plants are energetically expensive and are dependent on functional plastid metabolism. To understand how the stroma and the lumen proteomes adapt to low temperatures, we have taken a proteomic approach (difference gel electrophoresis) to identify proteins that changed in abundance in Arabidopsis chloroplasts during cold shock (1 day), and short- (10 days) and long-term (40 days) acclimation to 5°C. We show that cold shock (1 day) results in minimal change in the plastid proteomes, while short-term (10 days) acclimation results in major changes in the stromal but few changes in the lumen proteome. Long-term acclimation (40 days) results in modulation of the proteomes of both compartments, with new proteins appearing in the lumen and further modulations in protein abundance occurring in the stroma. We identify 43 differentially displayed proteins that participate in photosynthesis, other plastid metabolic functions, hormone biosynthesis and stress sensing and signal transduction. These findings not only provide new insights into the cold response and acclimation of Arabidopsis, but also demonstrate the importance of studying changes in protein abundance within the relevant cellular compartment.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
NATURVETENSKAP Biologi hsv//swe
NATURAL SCIENCES Biological Sciences hsv//eng
Acclimatization
Arabidopsis/genetics/*metabolism/physiology
Arabidopsis Proteins/classification/genetics/*metabolism
Chloroplasts/*metabolism/physiology
Cold
Electrophoresis; Gel; Two-Dimensional
Gene Expression Profiling
Photosynthesis
Proteomics
Signal Transduction
Time Factors

Schubert, MariaSödertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet(Swepub:sh)SHMABT (author)
Kieselbach, ThomasUmeå universitet,Kemiska institutionen(Swepub:umu)thskih04 (author)
Kleczkowski, LeszekUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)lekl0001 (author)
Gardeström, PerUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)pega0001 (author)
Schröder, WolfgangUmeå universitet,Kemiska institutionen,Umeå Plant Science Centre (UPSC)(Swepub:sh)SHWGSR (author)
Hurry, VaughanUmeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)(Swepub:umu)vahu0001 (author)
Umeå universitet / Université des Sciences et Technologies de Lille 1, FranceInstitutionen för livsvetenskaper (creator_code:org_t)

By the author/editor: Goulas, Estelle; Schubert, Maria; Kieselbach, Thom ...; Kleczkowski, Les ...; Gardeström, Per; Schröder, Wolfga ...; show more...; Hurry, Vaughan; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

By the university: Umeå University; Södertörn University; Karolinska Institutet

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