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Structure of photos...
Structure of photosystem II and substrate binding at room temperature
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- Young, Iris D. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Ibrahim, Mohamed (author)
- Humboldt Univ, Inst Biol, D-10099 Berlin, Germany.
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- Chatterjee, Ruchira (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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show more...
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- Gul, Sheraz (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Fuller, Franklin D. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Koroidov, Sergey (author)
- Umeå universitet,Kemiska institutionen,Umea Univ, Inst Kemi, Kemiskt Biol Ctr, S-90187 Umea, Sweden.
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- Brewster, Aaron S. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Tran, Rosalie (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Alonso-Mori, Roberto (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Kroll, Thomas (author)
- SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.;SLAC Natl Accelerator Lab, SSRL, Menlo Pk, CA 94025 USA.
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- Michels-Clark, Tara (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Laksmono, Hartawan (author)
- SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.
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- Sierra, Raymond G. (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.;SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.
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- Stan, Claudiu A. (author)
- SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.,SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Hussein, Rana (author)
- Humboldt Univ, Inst Biol, D-10099 Berlin, Germany.
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- Zhang, Miao (author)
- Humboldt Univ, Inst Biol, D-10099 Berlin, Germany.
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- Douthit, Lacey (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Kubin, Markus (author)
- Helmholtz Zentrum, Inst Methods & Instrumentat Synchrotron Radiat Re, D-14109 Berlin, Germany.
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- de Lichtenberg, Casper (author)
- Umeå universitet,Kemiska institutionen,Umea Univ, Inst Kemi, Kemiskt Biol Ctr, S-90187 Umea, Sweden.
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- Vo Pham, Long (author)
- Umeå universitet,Kemiska institutionen
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- Nilsson, Håkan (author)
- Umeå universitet,Kemiska institutionen,Umea Univ, Inst Kemi, Kemiskt Biol Ctr, S-90187 Umea, Sweden.
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- Cheah, Mun Hon (author)
- Umeå universitet,Kemiska institutionen,Umea Univ, Inst Kemi, Kemiskt Biol Ctr, S-90187 Umea, Sweden.
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- Shevela, Dmitriy (author)
- Umeå universitet,Kemiska institutionen,Umea Univ, Inst Kemi, Kemiskt Biol Ctr, S-90187 Umea, Sweden.
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- Saracini, Claudio (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Bean, Mackenzie A. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Seuffert, Ina (author)
- Humboldt Univ, Inst Biol, D-10099 Berlin, Germany.
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- Sokaras, Dimosthenis (author)
- SLAC Natl Accelerator Lab, SSRL, Menlo Pk, CA 94025 USA.
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- Weng, Tsu-Chien (author)
- SLAC Natl Accelerator Lab, SSRL, Menlo Pk, CA 94025 USA.;Ctr High Pressure Sci & Technol Adv Res, Shanghai 201203, Peoples R China.
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- Pastor, Ernest (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Weninger, Clemens (author)
- SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.
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- Fransson, Thomas (author)
- SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.
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- Lassalle, Louise (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Bräuer, Philipp (author)
- Univ Oxford, Dept Biochem, S Parks Rd, Oxford OX1 3QU, England.;Diamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.
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- Aller, Pierre (author)
- Diamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.
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- Docker, Peter T. (author)
- Diamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.
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- Andi, Babak (author)
- Brookhaven Natl Lab, Natl Synchrotron Light Source 2, Upton, NY 11973 USA.
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- Orville, Allen M. (author)
- Diamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.
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- Glownia, James M. (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Nelson, Silke (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Sikorski, Marcin (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Zhu, Diling (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Hunter, Mark S. (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Lane, Thomas J. (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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Aquila, Andy (author)
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- Koglin, Jason E. (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Robinson, Joseph (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Liang, Mengning (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Boutet, Sébastien (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Lyubimov, Artem Y. (author)
- Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA.;Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA.
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- Uervirojnangkoorn, Monarin (author)
- Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA.;Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA.
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- Moriarty, Nigel W. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Liebschner, Dorothee (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Afonine, Pavel V. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Waterman, David G. (author)
- STFC Rutherford Appleton Lab, Didcot OX11 0QX, Oxon, England.;Rutherford Appleton Lab, CCP4,Res Complex Harwell, Didcot OX11 0FA, Oxon, England.
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- Evans, Gwyndaf (author)
- Diamond Light Source Ltd, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England.
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- Wernet, Philippe (author)
- Helmholtz Zentrum, Inst Methods & Instrumentat Synchrotron Radiat Re, D-14109 Berlin, Germany.
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- Dobbek, Holger (author)
- Humboldt Univ, Inst Biol, D-10099 Berlin, Germany.
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- Weis, William I. (author)
- Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA.;Stanford Univ, Dept Photon Sci, Stanford, CA 94305 USA.;Stanford Univ, Dept Struct Biol, Stanford, CA 94305 USA.
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- Brunger, Axel T. (author)
- Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA.;Stanford Univ, Howard Hughes Med Inst, Stanford, CA 94305 USA.;Stanford Univ, Dept Photon Sci, Stanford, CA 94305 USA.;Stanford Univ, Dept Struct Biol, Stanford, CA 94305 USA.
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- Zwart, Petrus H. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Adams, Paul D. (author)
- Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA.
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- Zouni, Athina (author)
- Humboldt Univ, Inst Biol, D-10099 Berlin, Germany.
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- Messinger, Johannes (author)
- Uppsala universitet,Umeå universitet,Kemiska institutionen,Department of Chemistry, Molecular Biomimetics, Ångström Laboratory, Uppsala University,Molekylär biomimetik,Umea Univ, Inst Kemi, Kemiskt Biol Ctr, S-90187 Umea, Sweden.
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- Bergmann, Uwe (author)
- SLAC Natl Accelerator Lab, Stanford PULSE Inst, Menlo Pk, CA 94025 USA.
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- Sauter, Nicholas K. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Kern, Jan (author)
- SLAC Natl Accelerator Lab, LCLS, Menlo Pk, CA 94025 USA.
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- Yachandra, Vittal K. (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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- Yano, Junko (author)
- Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA.
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Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA 94720 USA Humboldt Univ, Inst Biol, D-10099 Berlin, Germany. (creator_code:org_t)
- 2016-11-21
- 2016
- English.
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In: Nature. - : Macmillan Publishers Ltd.. - 0028-0836 .- 1476-4687. ; 540:7633, s. 453-457
- Related links:
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https://europepmc.or...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Light-induced oxidation of water by photosystem II (PS II) in plants, algae and cyanobacteria has generated most of the dioxygen in the atmosphere. PS II, a membrane-bound multi-subunit pigment protein complex, couples the one-electron photochemistry at the reaction centre with the four-electron redox chemistry of water oxidation at the Mn4CaO5 cluster in the oxygen-evolving complex (OEC). Under illumination, the OEC cycles through five intermediate S-states (S0 to S4)1, in which S1 is the dark-stable state and S3 is the last semi-stable state before O–O bond formation and O2 evolution2,3. A detailed understanding of the O–O bond formation mechanism remains a challenge, and will require elucidation of both the structures of the OEC in the different S-states and the binding of the two substrate waters to the catalytic site4–6. Here we report the use of femtosecond pulses from an X-ray free electron laser (XFEL) to obtain damage-free, room temperature structures of dark-adapted (S1), two-flash illuminated (2F; S3-enriched), and ammonia-bound two-flash illuminated (2F-NH3; S3-enriched) PS II. Although the recent 1.95 Å resolution structure of PS II at cryogenic temperature using an XFEL7 provided a damage-free view of the S1 state, measurements at room temperature are required to study the structural landscape of proteins under functional conditions8,9, and also for in situ advancement of the S-states. To investigate the water-binding site(s), ammonia, a water analogue, has been used as a marker, as it binds to the Mn4CaO5 cluster in the S2 and S3 states10. Since the ammonia-bound OEC is active, the ammonia-binding Mn site is not a substrate water site10–13. This approach, together with a comparison of the native dark and 2F states, is used to discriminate between proposed O–O bond formation mechanisms.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
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- ref (subject category)
- art (subject category)
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Nature
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- By the author/editor
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Young, Iris D.
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Ibrahim, Mohamed
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Chatterjee, Ruch ...
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Gul, Sheraz
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Fuller, Franklin ...
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Koroidov, Sergey
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show more...
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Brewster, Aaron ...
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Tran, Rosalie
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Alonso-Mori, Rob ...
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Kroll, Thomas
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Michels-Clark, T ...
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Laksmono, Hartaw ...
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Sierra, Raymond ...
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Stan, Claudiu A.
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Hussein, Rana
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Zhang, Miao
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Douthit, Lacey
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Kubin, Markus
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de Lichtenberg, ...
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Vo Pham, Long
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Nilsson, Håkan
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Cheah, Mun Hon
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Shevela, Dmitriy
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Saracini, Claudi ...
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Bean, Mackenzie ...
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Seuffert, Ina
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Sokaras, Dimosth ...
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Weng, Tsu-Chien
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Pastor, Ernest
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Weninger, Clemen ...
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Fransson, Thomas
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Lassalle, Louise
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Bräuer, Philipp
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Aller, Pierre
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Docker, Peter T.
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Andi, Babak
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Orville, Allen M ...
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Glownia, James M ...
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Nelson, Silke
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Sikorski, Marcin
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Zhu, Diling
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Hunter, Mark S.
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Lane, Thomas J.
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Aquila, Andy
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Koglin, Jason E.
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Robinson, Joseph
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Liang, Mengning
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Boutet, Sébastie ...
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Lyubimov, Artem ...
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Uervirojnangkoor ...
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Moriarty, Nigel ...
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Liebschner, Doro ...
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Afonine, Pavel V ...
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Waterman, David ...
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Evans, Gwyndaf
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Wernet, Philippe
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Dobbek, Holger
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Weis, William I.
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Brunger, Axel T.
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Zwart, Petrus H.
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Adams, Paul D.
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Zouni, Athina
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Messinger, Johan ...
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Bergmann, Uwe
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Sauter, Nicholas ...
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Kern, Jan
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Yachandra, Vitta ...
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Yano, Junko
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
- Articles in the publication
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Nature
- By the university
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Umeå University
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Uppsala University