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Elimination of chan...
Elimination of chanel-forming activity by insertional inactivation of the p66 gene in Borrelia burgdorferi.
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- Pinne, Marija (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström
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Thein, Marcus (author)
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Denker, Katrin (author)
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Benz, Roland (author)
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Coburn, Jenifer (author)
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- Bergström, Sven (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Bergström
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(creator_code:org_t)
- Oxford University Press (OUP), 2007
- 2007
- English.
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In: FEMS Microbiol Lett. - : Oxford University Press (OUP). - 0378-1097 .- 1574-6968. ; 266, s. 241-249
- Related links:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Abstract P66 is a chromosomally encoded 66-kDa integral outer membrane protein of the Lyme disease agent Borrelia burgdorferi exhibiting channel-forming activity. Herein, we inactivated and subsequently complemented the p66 gene in the B31-A (WT) strain. The P66 protein was also inactivated in two other channel-forming protein mutant strains, P13-18 (Deltap13) and Deltabba01, and then compared with the channel-forming activities of wild-type and various p66 mutant strains. We further investigated the ion-selectivity of native, purified P66. In conclusion, we show that the porin activity of P66 is eliminated by insertional inactivation and that this activity can be rescued by gene complementation.
Keyword
- channel-forming activity
- P66
- porin
- insertional inactivation
- Borrelia burgdorferi
Publication and Content Type
- ref (subject category)
- art (subject category)
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