Detection of low levels of Escherichia coli by electrochemical impedance spectroscopy and singular value decomposition

Krupinska, Karolina (author)

Geladi, Paul (author): Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Skogens biomaterial och teknologi,Department of Forest Biomaterials and Technology,Corpus Data & Image Analysis AB

Vikholm-Lundin, Inger (author)

(creator_code:org_t)

Elsevier, 2017
2017
English.
In: Chemometrics and Intelligent Laboratory Systems. - : Elsevier. - 0169-7439 .- 1873-3239. ; 163, s. 49-54

Related links:: https://urn.kb.se/re...; show more...; https://doi.org/10.1...; https://res.slu.se/i...; show less...

Abstract Subject headings

The first steps are reported in the development of a new ultrasensitive electrochemical biosensor for detection of Escherichia coli (E. coli) in water. Two gold electrodes in a sandwich flow-cell were modified with E. coli polyclonal antibody and exposed to three different concentrations of E. coli. Electrochemical Impedance Spectroscopy was used in combination with Singular Value Decompostion of the complex numbers to monitor the interactions at the electrode surfaces. A linear regression line in the concentration range 10-1000 CFU⁎ml-1 was obtained without use of redox probes or metal nanoparticles for signal amplification.

By the author/editor: Krupinska, Karol ...; Geladi, Paul; Vikholm-Lundin, ...; Lindholm-Sethson ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

By the university: Umeå University; Swedish University of Agricultural Sciences

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