Search: onr:"swepub:oai:DiVA.org:umu-129267" >
Coordination of m(6...
Coordination of m(6)A mRNA Methylation and Gene Transcription by ZFP217 Regulates Pluripotency and Reprogramming.
-
- Aguilo, Francesca (author)
- Francesca Aguilo
-
Zhang, Fan (author)
-
Sancho, Ana (author)
-
show more...
-
Fidalgo, Miguel (author)
-
Di Cecilia, Serena (author)
-
Vashisht, Ajay (author)
-
Lee, Dung-Fang (author)
-
Chen, Chih-Hung (author)
-
Rengasamy, Madhumitha (author)
-
Andino, Blanca (author)
-
Jahouh, Farid (author)
-
Roman, Angel (author)
-
Krig, Sheryl R (author)
-
Wang, Rong (author)
-
Zhang, Weijia (author)
-
Wohlschlegel, James A (author)
-
Wang, Jianlong (author)
-
Walsh, Martin J (author)
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
-
In: Cell Stem Cell. - : Elsevier BV. - 1934-5909 .- 1875-9777. ; 17:6, s. 689-704
- Related links:
-
http://www.cell.com/...
-
show more...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- Epigenetic and epitranscriptomic networks have important functions in maintaining the pluripotency of embryonic stem cells (ESCs) and somatic cell reprogramming. However, the mechanisms integrating the actions of these distinct networks are only partially understood. Here we show that the chromatin-associated zinc finger protein 217 (ZFP217) coordinates epigenetic and epitranscriptomic regulation. ZFP217 interacts with several epigenetic regulators, activates the transcription of key pluripotency genes, and modulates N6-methyladenosine (m(6)A) deposition on their transcripts by sequestering the enzyme m(6)A methyltransferase-like 3 (METTL3). Consistently, Zfp217 depletion compromises ESC self-renewal and somatic cell reprogramming, globally increases m(6)A RNA levels, and enhances m(6)A modification of the Nanog, Sox2, Klf4, and c-Myc mRNAs, promoting their degradation. ZFP217 binds its own target gene mRNAs, which are also METTL3 associated, and is enriched at promoters of m(6)A-modified transcripts. Collectively, these findings shed light on how a transcription factor can tightly couple gene transcription to m(6)A RNA modification to ensure ESC identity.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Aguilo, Francesc ...
-
Zhang, Fan
-
Sancho, Ana
-
Fidalgo, Miguel
-
Di Cecilia, Sere ...
-
Vashisht, Ajay
-
show more...
-
Lee, Dung-Fang
-
Chen, Chih-Hung
-
Rengasamy, Madhu ...
-
Andino, Blanca
-
Jahouh, Farid
-
Roman, Angel
-
Krig, Sheryl R
-
Wang, Rong
-
Zhang, Weijia
-
Wohlschlegel, Ja ...
-
Wang, Jianlong
-
Walsh, Martin J
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Cell and Molecul ...
- Articles in the publication
-
Cell Stem Cell
- By the university
-
Umeå University