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Coordination of m(6...
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Aguilo, FrancescaFrancesca Aguilo
(author)
Coordination of m(6)A mRNA Methylation and Gene Transcription by ZFP217 Regulates Pluripotency and Reprogramming.
- Article/chapterEnglish2015
Publisher, publication year, extent ...
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Elsevier BV,2015
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-129267
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-129267URI
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https://doi.org/10.1016/j.stem.2015.09.005DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Epigenetic and epitranscriptomic networks have important functions in maintaining the pluripotency of embryonic stem cells (ESCs) and somatic cell reprogramming. However, the mechanisms integrating the actions of these distinct networks are only partially understood. Here we show that the chromatin-associated zinc finger protein 217 (ZFP217) coordinates epigenetic and epitranscriptomic regulation. ZFP217 interacts with several epigenetic regulators, activates the transcription of key pluripotency genes, and modulates N6-methyladenosine (m(6)A) deposition on their transcripts by sequestering the enzyme m(6)A methyltransferase-like 3 (METTL3). Consistently, Zfp217 depletion compromises ESC self-renewal and somatic cell reprogramming, globally increases m(6)A RNA levels, and enhances m(6)A modification of the Nanog, Sox2, Klf4, and c-Myc mRNAs, promoting their degradation. ZFP217 binds its own target gene mRNAs, which are also METTL3 associated, and is enriched at promoters of m(6)A-modified transcripts. Collectively, these findings shed light on how a transcription factor can tightly couple gene transcription to m(6)A RNA modification to ensure ESC identity.
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Zhang, Fan
(author)
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Sancho, Ana
(author)
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Fidalgo, Miguel
(author)
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Di Cecilia, Serena
(author)
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Vashisht, Ajay
(author)
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Lee, Dung-Fang
(author)
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Chen, Chih-Hung
(author)
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Rengasamy, Madhumitha
(author)
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Andino, Blanca
(author)
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Jahouh, Farid
(author)
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Roman, Angel
(author)
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Krig, Sheryl R
(author)
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Wang, Rong
(author)
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Zhang, Weijia
(author)
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Wohlschlegel, James A
(author)
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Wang, Jianlong
(author)
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Walsh, Martin J
(author)
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Francesca Aguilo
(creator_code:org_t)
Related titles
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In:Cell Stem Cell: Elsevier BV17:6, s. 689-7041934-59091875-9777
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- By the author/editor
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Aguilo, Francesc ...
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Zhang, Fan
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Sancho, Ana
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Fidalgo, Miguel
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Di Cecilia, Sere ...
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Vashisht, Ajay
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show more...
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Lee, Dung-Fang
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Chen, Chih-Hung
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Rengasamy, Madhu ...
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Andino, Blanca
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Jahouh, Farid
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Roman, Angel
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Krig, Sheryl R
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Wang, Rong
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Zhang, Weijia
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Wohlschlegel, Ja ...
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Wang, Jianlong
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Walsh, Martin J
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
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Cell Stem Cell
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Umeå University