Sökning: onr:"swepub:oai:DiVA.org:umu-129272" >
RBM5-AS1 Is Critica...
RBM5-AS1 Is Critical for Self-Renewal of Colon Cancer Stem-like Cells
-
Di Cecilia, Serena (författare)
-
Zhang, Fan (författare)
-
Sancho, Ana (författare)
-
visa fler...
-
Li, SiDe (författare)
-
- Aguiló, Francesca (författare)
- Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York; Department of Pediatrics, Icahn School of Medicine at Mount Sinai, New York; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York,Francesca Aguilo
-
Sun, Yifei (författare)
-
Rengasamy, Madhumitha (författare)
-
Zhang, Weijia (författare)
-
Del Vecchio, Luigi (författare)
-
Salvatore, Francesco (författare)
-
Walsh, Martin J. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2016
- 2016
- Engelska.
-
Ingår i: Cancer Research. - 0008-5472 .- 1538-7445. ; 76:19, s. 5615-5627
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Cancer-initiating cells (CIC) undergo asymmetric growth patterns that increase phenotypic diversity and drive selection for chemotherapeutic resistance and tumor relapse. WNT signaling is a hallmark of colon CIC, often caused by APC mutations, which enable activation of β-catenin and MYC Accumulating evidence indicates that long noncoding RNAs (lncRNA) contribute to the stem-like character of colon cancer cells. In this study, we report enrichment of the lncRNA RBM5-AS1/LUST during sphere formation of colon CIC. Its silencing impaired WNT signaling, whereas its overexpression enforced WNT signaling, cell growth, and survival in serum-free media. RBM5-AS1 has been little characterized previously, and we determined it to be a nuclear-retained transcript that selectively interacted with β-catenin. Mechanistic investigations showed that silencing or overexpression of RBM5-AS1 caused a respective loss or retention of β-catenin from TCF4 complexes bound to the WNT target genes SGK1, YAP1, and MYC Our work suggests that RBM5-AS1 activity is critical for the functional enablement of colon cancer stem-like cells. Furthermore, it defines the mechanism of action of RBM5-AS1 in the WNT pathway via physical interactions with β-catenin, helping organize transcriptional complexes that sustain colon CIC function.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Di Cecilia, Sere ...
-
Zhang, Fan
-
Sancho, Ana
-
Li, SiDe
-
Aguiló, Francesc ...
-
Sun, Yifei
-
visa fler...
-
Rengasamy, Madhu ...
-
Zhang, Weijia
-
Del Vecchio, Lui ...
-
Salvatore, Franc ...
-
Walsh, Martin J.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
- Artiklar i publikationen
-
Cancer Research
- Av lärosätet
-
Umeå universitet