A host-derived pentapeptide affecting adhesion, proliferation and local pH in Streptococcus-Actinomyces biofilm communities.

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Drobni, MirvaUmeå universitet,Kariologi (author)

A host-derived pentapeptide affecting adhesion, proliferation and local pH in Streptococcus-Actinomyces biofilm communities.

Article/chapterEnglish2006

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2006
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LIBRIS-ID:oai:DiVA.org:umu-12949
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-12949URI

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Language:English
Summary in:English

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SwePubSwePub

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Subject category:pop swepub-contenttype
Subject category:for swepub-publicationtype

Notes

Salivary proline-rich proteins (PRPs) attach commensal Actinomyces and Streptococcus species to teeth. Here, gel filtration, mass spectrometry and Edman degradation were applied to show the release of a pentapeptide, RGRPQ, from PRP-1 upon proteolysis by Streptococcus gordonii. Moreover, synthetic RGRPQ and derivatives were used to investigate associated innate properties and responsible motifs. The RGRPQ peptide increased 2.5-fold the growth rate of S. gordonii via a Q-dependent sequence motif, and selectively stimulated oral colonization of this organism in a rat model in vivo. By contrast, growth of Streptococcus mutans, implicated in caries, was unaffected. While the entire RGRPQ sequence was required to block sucrose-induced pH-decrease by S. gordonii and S. mutans, the N-terminal Arg residue mediated pH-increase (i.e. ammonia production) by S. gordonii alone (which exhibits Arg catabolism to ammonia). Strains of commensal viridans streptococci exhibited PRP degradation and Arg catabolism, while cariogenic species did not. The RGRPQ peptide mediated via a differential Q-dependent sequence motif, adhesion inhibition and desorption of PRP-1-binding strains of A. naeslundii genospecies 2 (5 out of 10 strains) but not of S. gordonii (n=5). The inhibitable A. naeslundii strains alone displayed the same binding profile as S. gordonii to hybrid peptides terminating in RGRPQ or GQSPQ, derived from the middle or C-terminal segments of PRP-1. The present findings indicate the presence of a host-bacteria interaction where a host peptide released by bacterial proteolysis affects key properties in biofilm formation.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Odontologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Dentistry hsv//eng
ODONTOLOGY
ODONTOLOGI
Cariology
Cariologi

Added entries (persons, corporate bodies, meetings, titles ...)

Li, Tong (author)
Kruger, C (author)
Loimaranta, V (author)
Kilian, M (author)
Hammarström, L (author)
Jörnvall, H (author)
Bergman, T (author)
Strömberg, Nicklas (author)
Umeå universitetKariologi (creator_code:org_t)

Related titles

In:Infection and immunity

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By the author/editor: Drobni, Mirva; Li, Tong; Kruger, C; Loimaranta, V; Kilian, M; Hammarström, L; show more...; Jörnvall, H; Bergman, T; Strömberg, Nickl ...; show less...

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dentistry

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By the university: Umeå University

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