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Purification and ch...
Purification and characterization of recombinant human bile salt-stimulated lipase expressed in milk of transgenic cloned cows
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Wang, Yuhang (author)
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Ding, Fangrong (author)
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Wang, Tao (author)
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Liu, Wenjie (author)
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- Lindquist, Susanne (author)
- Umeå universitet,Pediatrik
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- Hernell, Olle (author)
- Umeå universitet,Pediatrik
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Wang, Jianwu (author)
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Li, Jing (author)
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Li, Ling (author)
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Zhao, Yaofeng (author)
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Dai, Yunping (author)
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Li, Ning (author)
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(creator_code:org_t)
- 2017-05-05
- 2017
- English.
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In: PLOS ONE. - : PUBLIC LIBRARY SCIENCE. - 1932-6203. ; 12:5
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Bile salt-stimulated lipase (BSSL) is a lipolytic digestive enzyme with broad substrate specificity secreted from exocrine pancreas into the intestinal lumen in all species and from the lactating mammary gland into the milk of some species, notably humans but not cows. BSSL in breast milk facilitates digestion and absorption of milk fat and promotes growth of small for gestational age preterm infants. Thus, purified recombinant human BSSL (rhBSSL) can be used for treatment of patients with fat malabsorption and expressing rhBSSL in the milk of transgenic cloned cows would therefore be a mean to meet a medical need. In the present study, a vector pBAC-hLF-hBSSL was constructed, which efficiently expressed active rhBSSL in milk of transgenic cloned cows to a concentration of 9.8 mg/ml. The rhBSSL purified from cow milk had the same enzymatic activity, N-terminal amino acid sequence, amino acid composition and isoelectric point and similar physicochemical characteristics as human native BSSL. Our study supports the use of transgenic cattle for the cost-competitive, large-scale production of therapeutic rhBSSL.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Wang, Yuhang
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Ding, Fangrong
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Wang, Tao
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Liu, Wenjie
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Lindquist, Susan ...
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Hernell, Olle
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show more...
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Wang, Jianwu
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Li, Jing
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Li, Ling
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Zhao, Yaofeng
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Dai, Yunping
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Li, Ning
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medicinal Chemis ...
- Articles in the publication
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PLOS ONE
- By the university
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Umeå University