Mediator binds to boundaries of chromosomal interaction domains and to proteins involved in DNA looping, RNA metabolism, chromatin remodeling, and actin assembly

Chereji, Razvan V. (author)

Bharatula, Vasudha (author)

Elfving, Nils (author): Umeå universitet,Institutionen för medicinsk kemi och biofysik

Blomberg, Jeanette (author): Umeå universitet,Institutionen för medicinsk kemi och biofysik

Larsson, Miriam (author): Umeå universitet,Institutionen för medicinsk kemi och biofysik

Morozov, Alexandre V. (author)

Broach, James R. (author)

Björklund, Stefan (author): Umeå universitet,Institutionen för medicinsk kemi och biofysik

(creator_code:org_t)

2017-05-30
2017
English.
In: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 45:15, s. 8806-8821

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Abstract Subject headings

Mediator is a multi-unit molecular complex that plays a key role in transferring signals from transcriptional regulators to RNA polymerase II in eukaryotes. We have combined biochemical purification of the Sac-charomyces cerevisiae Mediator from chromatin with chromatin immunoprecipitation in order to reveal Mediator occupancy on DNA genome-wide, and to identify proteins interacting specifically with Mediator on the chromatin template. Tandem mass spectrometry of proteins in immunoprecipitates of mediator complexes revealed specific interactions between Mediator and the RSC, Arp2/Arp3, CPF, CF 1A and Lsm complexes in chromatin. These factors are primarily involved in chromatin remodeling, actin assembly, mRNA 3'-end processing, gene looping and mRNA decay, but they have also been shown to enter the nucleus and participate in Pol II transcription. Moreover, we have found that Mediator, in addition to binding Pol II promoters, occupies chromosomal interacting domain (CID) boundaries and that Mediator in chromatin associates with proteins that have been shown to interact with CID boundaries, such as Sth1, Ssu72 and histone H4. This suggests that Mediator plays a significant role in higher-order genome organization.

By the author/editor: Chereji, Razvan ...; Bharatula, Vasud ...; Elfving, Nils; Blomberg, Jeanet ...; Larsson, Miriam; Morozov, Alexand ...; show more...; Broach, James R.; Björklund, Stefa ...; show less...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Biochemistry and ...

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