Organellar gene transcription and early seedling development are affected in the rpoT;2 mutant of Arabidopsis.

Baba, Kyoko (author)

Schmidt, Julien (author)

Espinosa-Ruiz, Ana (author)

Villarejo, Arsenio (author)

Shiina, Takashi (author)

Gardeström, Per (author): Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)

Sane, Aniruddha P (author)

Bhalerao, Rishikesh P (author): Umeå universitet,Umeå Plant Science Centre (UPSC)

(creator_code:org_t)

Related links:: http://www.ncbi.nlm....; show more...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

An Arabidopsis mutant that exhibited reduced root length was isolated from a population of activation-tagged T-DNA insertion lines in a screen for aberrant root growth. This mutant also exhibited reduced hypocotyl length as well as a delay in greening and altered leaf shape. Molecular genetic analysis of the mutant indicated a single T-DNA insertion in the gene RpoT;2 encoding a homolog of the phage-type RNA polymerase (RNAP), that is targeted to both mitochondria and plastids. A second T-DNA-tagged allele also showed a similar phenotype. The mutation in RpoT;2 affected the light-induced accumulation of several plastid mRNAs and proteins and resulted in a lower photosynthetic efficiency. In contrast to the alterations in the plastid gene expression, no major effect of the rpoT;2 mutation on the accumulation of examined mitochondrial gene transcripts and proteins was observed. The rpoT;2 mutant exhibited tissue-specific alterations in the transcript levels of two other organelle-directed nuclear-encoded RNAPs, RpoT;1 and RpoT;3. This suggests the existence of cross-talk between the regulatory pathways of the three RNAPs through organelle to nucleus communication. These data provide an important information on a role of RpoT;2 in plastid gene expression and early plant development.

By the author/editor: Baba, Kyoko; Schmidt, Julien; Espinosa-Ruiz, A ...; Villarejo, Arsen ...; Shiina, Takashi; Gardeström, Per; show more...; Sane, Aniruddha ...; Bhalerao, Rishik ...; show less...

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