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Inhibition of curli...
Inhibition of curli assembly and Escherichia coli biofilm formation by the human systemic amyloid precursor transthyretin
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Jain, Neha (author)
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- Ådén, Jörgen (author)
- Umeå universitet,Kemiska institutionen
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Nagamatsu, Kanna (author)
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Evans, Margery L. (author)
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Li, Xinyi (author)
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McMichael, Brennan (author)
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Ivanova, Magdalena I. (author)
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- Almqvist, Fredrik (author)
- Umeå universitet,Kemiska institutionen
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Buxbaum, Joel N. (author)
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Chapman, Matthew R. (author)
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(creator_code:org_t)
- 2017-10-30
- 2017
- English.
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 114:46, s. 12184-12189
- Related links:
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https://www.pnas.org...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- During biofilm formation, Escherichia coli and other Enterobacteriaceae produce an extracellular matrix consisting of curli amyloid fibers and cellulose. The precursor of curli fibers is the amyloidogenic protein CsgA. The human systemic amyloid precursor protein transthyretin (TTR) is known to inhibit amyloid-β (Aβ) aggregation in vitro and suppress the Alzheimer’s-like phenotypes in a transgenic mouse model of Aβ deposition. We hypothesized that TTR might have broad antiamyloid activity because the biophysical properties of amyloids are largely conserved across species and kingdoms. Here, we report that both human WT tetrameric TTR (WT-TTR) and its engineered nontetramer-forming monomer (M-TTR, F87M/L110M) inhibit CsgA amyloid formation in vitro, with M-TTR being the more efficient inhibitor. Preincubation of WT-TTR with small molecules that occupy the T4 binding site eliminated the inhibitory capacity of the tetramer; however, they did not significantly compromise the ability of M-TTR to inhibit CsgA amyloidogenesis. TTR also inhibited amyloid-dependent biofilm formation in two different bacterial species with no apparent bactericidal or bacteriostatic effects. These discoveries suggest that TTR is an effective antibiofilm agent that could potentiate antibiotic efficacy in infections associated with significant biofilm formation.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Amyloids
- CsgA
- Transthyretin
- Biofilms
- Curli
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Jain, Neha
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Ådén, Jörgen
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Nagamatsu, Kanna
-
Evans, Margery L ...
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Li, Xinyi
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McMichael, Brenn ...
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show more...
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Ivanova, Magdale ...
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Almqvist, Fredri ...
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Buxbaum, Joel N.
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Chapman, Matthew ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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Proceedings of t ...
- By the university
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Umeå University