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Covalently electrog...
Covalently electrografted carboxyphenyl layers onto gold surface serving as a platform for the construction of an immunosensor for detection of methotrexate
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- Phal, Sereilakhena (author)
- Umeå universitet,Kemiska institutionen
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Shatri, Besart (author)
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Berisha, Avni (author)
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- Geladi, Paul (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Skogens biomaterial och teknologi,Department of Forest Biomaterials and Technology
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- Lindholm-Sethson, Britta (author)
- Umeå universitet,Kemiska institutionen
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Tesfalidet, Solomon (author)
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(creator_code:org_t)
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- Elsevier, 2018
- 2018
- English.
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In: Journal of Electroanalytical Chemistry. - : Elsevier. - 1572-6657 .- 1873-2569. ; 812, s. 235-243
- Related links:
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http://www.sciencedi...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Subject headings
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- Abstract The classical way to modify a gold electrode surface for immunosensor development is through self-assembly of functionalised thiols for subsequent attachment of antibodies. Here is described a new pathway for surface modification using a diazonium salt-based immunosensor for detection of methotrexate (MTX) with electrochemical immittance spectroscopy (EIS). The diazonium salt, 4-carboxybenzenediazonium tetrafluoroborate was synthesized using a diazotization reaction and characterized by IR and cyclic voltammetry (CV). The Au electrode was electrografted with diazonium and used for anti-MTX antibody immobilization. The EIS detection of MTX was studied with and without application of redox probe; Fe(CN)63−/4−. MTX could not be detected using Fe(CN)63−/4−as redox probe, whereas EIS measurements without redox probe and analysis of the data with singular decomposition (SVD) gave good results. A multivariate calibration model showed good linear behavior on a logarithmic scale with a detection limit for MTX of 7×10−12molL−1.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- 4-carboxybenzenediazonium tetrafluoroborate
- Gold electrode
- Antibody
- Immunosensor
- Singular value decomposition
- Cyclic voltammetry
- Chronoamperometry
- Electrochemical impedance spectroscopy
Publication and Content Type
- ref (subject category)
- art (subject category)
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