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Functional role of ...
Functional role of the type 1 pilus rod structure in mediating host-pathogen interactions
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Spaulding, Caitlin N. (author)
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Schreiber, Henry Louis (author)
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Zheng, Weili (author)
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Dodson, Karen W. (author)
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Hazen, Jennie E. (author)
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Conover, Matt S. (author)
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Wang, Fengbin (author)
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- Svenmarker, Pontus (author)
- Umeå universitet,Institutionen för fysik
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Luna-Rico, Areli (author)
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Francetic, Olivera (author)
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- Andersson, Magnus (author)
- Umeå universitet,Institutionen för fysik,The Biophysics and Biophotonics group
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Hultgren, Scott (author)
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Egelman, Edward H. (author)
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(creator_code:org_t)
- eLife Sciences Publications, 2018
- 2018
- English.
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In: eLIFE. - : eLife Sciences Publications. - 2050-084X. ; 7
- Related links:
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https://doi.org/10.7...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.7...
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Abstract
Subject headings
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- Uropathogenic E. coli (UPEC), which cause urinary tract infections (UTI), utilize type 1 pili, a chaperone usher pathway (CUP) pilus, to cause UTI and colonize the gut. The pilus rod, comprised of repeating FimA subunits, provides a structural scaffold for displaying the tip adhesin, FimH. We solved the 4.2 Å resolution structure of the type 1 pilus rod using cryo-electron microscopy. Residues forming the interactive surfaces that determine the mechanical properties of the rod were maintained by selection based on a global alignment of fimA sequences. We identified mutations that did not alter pilus production in vitro but reduced the force required to unwind the rod. UPEC expressing these mutant pili were significantly attenuated in bladder infection and intestinal colonization in mice. This study elucidates an unappreciated functional role for the molecular spring-like property of type 1 pilus rods in host-pathogen interactions and carries important implications for other pilus-mediated diseases.
Subject headings
- NATURVETENSKAP -- Biologi -- Strukturbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Structural Biology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Spaulding, Caitl ...
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Schreiber, Henry ...
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Zheng, Weili
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Dodson, Karen W.
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Hazen, Jennie E.
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Conover, Matt S.
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show more...
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Wang, Fengbin
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Svenmarker, Pont ...
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Luna-Rico, Areli
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Francetic, Olive ...
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Andersson, Magnu ...
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Hultgren, Scott
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Egelman, Edward ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Structural Biolo ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Other Physics To ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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eLIFE
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Umeå University