Transcriptome for photobiological hydrogen production induced by sulfur deprivation in the green algaChlamydomonas reinhardtii

• Photobiological hydrogen production using microalgae is being developed into a promising clean fuel stream for the future. In this study, microarray analyses were used to obtain global expression profiles of mRNA abundance in the green alga Chlamydomonas reinhardtii at different time points before the onset and during the course of sulfur-depleted hydrogen production. These studies were followed by real-time quantitative reverse transcription-PCR and protein analyses. The present work provides new insights into photosynthesis, sulfur acquisition strategies, and carbon metabolism-related gene expression during sulfur-induced hydrogen production. A general trend toward repression of transcripts encoding photosynthetic genes was observed. In contrast to all other LHCBM genes, the abundance of the LHCBM9 transcript (encoding a major light-harvesting polypeptide) and its protein was strongly elevated throughout the experiment. This suggests a major remodeling of the photosystem II light-harvesting complex as well as an important function of LHCBM9 under sulfur starvation and photobiological hydrogen production. This paper presents the first global transcriptional analysis of C. reinhardtii before, during, and after photobiological hydrogen production under sulfur deprivation.

Subject headings

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Keyword

Algal Proteins/genetics/metabolism

Amino Acids/metabolism

Chlorophyta/*genetics/*metabolism/radiation effects

*Gene Expression Profiling

Hydrogen/*metabolism

Light-Harvesting Protein Complexes/genetics/metabolism

Molecular Sequence Data

Oligonucleotide Array Sequence Analysis

*Photosynthesis

Protein Biosynthesis

Sulfur/*metabolism

Transcription

Genetic

Publication and Content Type

ref (subject category)

art (subject category)