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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 02668naa a2200385 4500 | |
| 001 | oai:DiVA.org:umu-147475 | |
| 003 | SwePub | |
| 008 | 180504s2018 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1474752 URI |
| 024 | 7 | a https://doi.org/10.1007/s11099-017-0758-42 DOI |
| 040 | a (SwePub)umu | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Tikhonov, K.4 aut |
| 245 | 1 0 | a Quantification of bound bicarbonate in photosystem II |
| 264 | 1 | b Institute of Experimental Botany,c 2018 |
| 338 | a print2 rdacarrier | |
| 520 | a In this study, we presented a new approach for quantification of bicarbonate (HCO3-) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and O-18-labelling, excludes the possibility of "non-accounted" HCO3- by avoiding (1) the employment of formate for removal of HCO3- from PSII, and (2) the extremely low concentrations of HCO3-/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3-, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 +/- 0.1 HCO3- are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3- molecule as ligand to the non-heme iron of PSII, while unbound HCO3- optimizes the water-splitting reactions by acting as a mobile proton shuttle. | |
| 650 | 7 | a NATURVETENSKAPx Biologix Biofysik0 (SwePub)106032 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Biophysics0 (SwePub)106032 hsv//eng |
| 653 | a hydrogen carbonate | |
| 653 | a inorganic carbon | |
| 653 | a mass spectrometry | |
| 653 | a Mn-stabilizing protein | |
| 653 | a non-heme iron | |
| 653 | a ygen-evolving complex | |
| 700 | 1 | a Shevela, Dmitryu Umeå universitet,Kemiska institutionen4 aut0 (Swepub:umu)dmsh0004 |
| 700 | 1 | a Klimov, V. V.4 aut |
| 700 | 1 | a Messinger, Johannesu Umeå universitet,Kemiska institutionen,Department of Chemistry, Molecular Biomimetics, Ångström Laboratory, Uppsala University, Uppsala, Sweden4 aut0 (Swepub:umu)jome0007 |
| 710 | 2 | a Umeå universitetb Kemiska institutionen4 org |
| 773 | 0 | t Photosynthetica (Praha)d : Institute of Experimental Botanyg 56:1, s. 210-216q 56:1<210-216x 0300-3604x 1573-9058 |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-147475 |
| 856 | 4 8 | u https://doi.org/10.1007/s11099-017-0758-4 |
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