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Quantitative Proteo...
Quantitative Proteomics Identifies Serum Response Factor Binding Protein 1 as a Host Factor for Hepatitis C Virus Entry
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- Gerold, Gisa, 1979- (author)
- Gisa Gerold
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Meissner, Felix (author)
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Bruening, Janina (author)
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Welsch, Kathrin (author)
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Perin, Paula M (author)
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Baumert, Thomas F (author)
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Vondran, Florian W (author)
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Kaderali, Lars (author)
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Marcotrigiano, Joseph (author)
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Khan, Abdul G (author)
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Mann, Matthias (author)
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Rice, Charles M (author)
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Pietschmann, Thomas (author)
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- English.
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In: Cell Reports. - : Elsevier BV. - 2211-1247. ; 12:5, s. 864-878
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http://www.cell.com/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- Hepatitis C virus (HCV) enters human hepatocytes through a multistep mechanism involving, among other host proteins, the virus receptor CD81. How CD81 governs HCV entry is poorly characterized, and CD81 protein interactions after virus binding remain elusive. We have developed a quantitative proteomics protocol to identify HCV-triggered CD81 interactions and found 26 dynamic binding partners. At least six of these proteins promote HCV infection, as indicated by RNAi. We further characterized serum response factor binding protein 1 (SRFBP1), which is recruited to CD81 during HCV uptake and supports HCV infection in hepatoma cells and primary human hepatocytes. SRFBP1 facilitates host cell penetration by all seven HCV genotypes, but not of vesicular stomatitis virus and human coronavirus. Thus, SRFBP1 is an HCV-specific, pan-genotypic host entry factor. These results demonstrate the use of quantitative proteomics to elucidate pathogen entry and underscore the importance of host protein-protein interactions during HCV invasion.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Gerold, Gisa, 19 ...
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Meissner, Felix
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Bruening, Janina
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Welsch, Kathrin
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Perin, Paula M
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Baumert, Thomas ...
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show more...
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Vondran, Florian ...
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Kaderali, Lars
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Marcotrigiano, J ...
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Khan, Abdul G
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Mann, Matthias
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Rice, Charles M
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Pietschmann, Tho ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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Cell Reports
- By the university
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Umeå University