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Germ line insertion...
Germ line insertions of moloney murine leukemia virus in the TLL mouse causes site-specific differences in lymphoma/leukemia frequency and tumor immunophenotype
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- Johansson, Ann-Sofie, 1967- (author)
- Umeå universitet,Medicinsk och klinisk genetik
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- Eriksson, Maria (author)
- Umeå universitet,Medicinsk och klinisk genetik
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- Norén-Nyström, Ulrika (author)
- Umeå universitet,Medicinsk och klinisk genetik
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- Larefalk, Åsa (author)
- Umeå universitet,Medicinsk och klinisk genetik
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Eriksson, Björn (author)
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- Holmberg, Dan (author)
- Umeå universitet,Medicinsk och klinisk genetik
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Vierimaa, O (author)
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Aittomaki, K (author)
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Pukkala, E (author)
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Launonen, V (author)
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Aaltonen, L A (author)
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(creator_code:org_t)
- 2006
- 2006
- English.
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In: Anticancer Research. - 0250-7005 .- 1791-7530. ; 26:4B, s. 2873-2878
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Background: Moloney murine leukemia virus (Mo-MLV) has proven valuable for studies of the pathogenesis of malignant lymphoma. Inoculation of newborn mice induces T cell lymphoma with 100% incidence. The TLL (T cell lymphoma/leukemia)-strain was previously established and was shown to spontaneously develop T cell lymphoma at high frequency. Materials and Methods: Differential screening of cDNA libraries was performed to discover an involvement of Mo-MLV and genomic sequencing was used to identify the chromosomal position of Mo-MLV proviral integration sites. Immunophenotypes of the tumors were established by flow cytometry. Disease frequency curves were created according to the Kaplan-Meier method. Results: Two independent Mo-MLV germ line integrations were characterized on chromosomes 2 and 14, giving rise to two substrains of mice denoted TLL-2 and TLL-14. The chromosomal position of the integrated provirus affected the frequency of disease, as well as the immunophenotype of the tumors. Conclusion: The data suggest that factors influencing the transcriptional activity of the chromosomal regions, leading to differences in proviral expression, could underlie the observed difference in tumor frequency.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Animals
- CD4-Positive T-Lymphocytes/immunology
- CD8-Positive T-Lymphocytes/immunology
- Immunophenotyping
- Leukemia; T-Cell/*genetics/immunology/*virology
- Lymphoma; T-Cell/*genetics/immunology/*virology
- Mice
- Mice; Inbred C57BL
- Moloney murine leukemia virus/*genetics
- Virus Integration
- Oncology
- Onkologi
- Oncology
- onkologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Johansson, Ann-S ...
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Eriksson, Maria
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Norén-Nyström, U ...
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Larefalk, Åsa
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Eriksson, Björn
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Holmberg, Dan
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show more...
-
Vierimaa, O
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Aittomaki, K
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Pukkala, E
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Launonen, V
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Aaltonen, L A
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
- Articles in the publication
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Anticancer Resea ...
- By the university
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Umeå University