Germ line insertions of moloney murine leukemia virus in the TLL mouse causes site-specific differences in lymphoma/leukemia frequency and tumor immunophenotype

Johansson, Ann-Sofie,1967-Umeå universitet,Medicinsk och klinisk genetik (author)

Background: Moloney murine leukemia virus (Mo-MLV) has proven valuable for studies of the pathogenesis of malignant lymphoma. Inoculation of newborn mice induces T cell lymphoma with 100% incidence. The TLL (T cell lymphoma/leukemia)-strain was previously established and was shown to spontaneously develop T cell lymphoma at high frequency. Materials and Methods: Differential screening of cDNA libraries was performed to discover an involvement of Mo-MLV and genomic sequencing was used to identify the chromosomal position of Mo-MLV proviral integration sites. Immunophenotypes of the tumors were established by flow cytometry. Disease frequency curves were created according to the Kaplan-Meier method. Results: Two independent Mo-MLV germ line integrations were characterized on chromosomes 2 and 14, giving rise to two substrains of mice denoted TLL-2 and TLL-14. The chromosomal position of the integrated provirus affected the frequency of disease, as well as the immunophenotype of the tumors. Conclusion: The data suggest that factors influencing the transcriptional activity of the chromosomal regions, leading to differences in proviral expression, could underlie the observed difference in tumor frequency.

Eriksson, MariaUmeå universitet,Medicinsk och klinisk genetik (author)
Norén-Nyström, UlrikaUmeå universitet,Medicinsk och klinisk genetik(Swepub:umu)kagr0007 (author)
Larefalk, ÅsaUmeå universitet,Medicinsk och klinisk genetik(Swepub:umu)asla0002 (author)
Eriksson, Björn (author)
Holmberg, DanUmeå universitet,Medicinsk och klinisk genetik(Swepub:umu)daho0001 (author)
Vierimaa, O (author)
Aittomaki, K (author)
Pukkala, E (author)
Launonen, V (author)
Aaltonen, L A (author)
Umeå universitetMedicinsk och klinisk genetik (creator_code:org_t)

By the author/editor: Johansson, Ann-S ...; Eriksson, Maria; Norén-Nyström, U ...; Larefalk, Åsa; Eriksson, Björn; Holmberg, Dan; show more...; Vierimaa, O; Aittomaki, K; Pukkala, E; Launonen, V; Aaltonen, L A; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cancer and Oncol ...

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