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Control of Bacteria...
Control of Bacterial Virulence through the Peptide Signature of the Habitat
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Krypotou, Emilia (author)
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Scortti, Mariela (author)
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- Grundström, Christin (author)
- Umeå universitet,Kemiska institutionen,Umeå Centre for Microbial Research (UCMR)
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- Oelker, Melanie (author)
- Umeå universitet,Kemiska institutionen,Umeå Centre for Microbial Research (UCMR)
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Luisi, Ben F. (author)
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- Sauer-Eriksson, A. Elisabeth (author)
- Umeå universitet,Kemiska institutionen,Umeå Centre for Microbial Research (UCMR)
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Vazquez-Boland, Jose (author)
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(creator_code:org_t)
- Elsevier, 2019
- 2019
- English.
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In: Cell Reports. - : Elsevier. - 2211-1247. ; 26:7, s. 1815-1827
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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http://www.cell.com/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
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- To optimize fitness, pathogens selectively activate their virulence program upon host entry. Here, we report that the facultative intracellular bacterium Listeria monocytogenes exploits exogenous oligopeptides, a ubiquitous organic N source, to sense the environment and control the activity of its virulence transcriptional activator, PrfA. Using a genetic screen in adsorbent- treated ( PrfA-inducing) medium, we found that PrfA is functionally regulated by the balance between activating and inhibitory nutritional peptides scavenged via the Opp transport system. Activating peptides provide essential cysteine precursor for the PrfA-inducing cofactor glutathione ( GSH). Non-cysteine-containing peptides cause promiscuous PrfA inhibition. Biophysical and co-crystallization studies reveal that peptides inhibit PrfA through steric blockade of the GSH binding site, a regulation mechanism directly linking bacterial virulence and metabolism. L. monocytogenes mutant analysis in macrophages and our functional data support a model in which changes in the balance of antagonistic Oppimported oligopeptides promote PrfA induction intra-cellularly and PrfA repression outside the host.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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