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Haemolysin II is a ...
Haemolysin II is a Bacillus cereus virulence factor that induces apoptosis of macrophages
- Article/chapterEnglish2011
Publisher, publication year, extent ...
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2010-12-20
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Hindawi Limited,2011
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:umu-156947
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-156947URI
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https://doi.org/10.1111/j.1462-5822.2010.01522.xDOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation despite the recruitment of phagocytic cells. The precise mechanisms and the bacterial factors allowing B. cereus to circumvent host immune responses remain to be elucidated. We have previously shown that B. cereus induces macrophage cell death by an unknown mechanism. Here we identified the toxic component from the B. cereus supernatant. We report that Haemolysin II (HlyII) provokes macrophage cell death by apoptosis through its pore-forming activity. The HlyII-induced apoptotic pathway is caspase 3 and 8 dependent, thus most likely mediated by the death receptor pathway. Using insects and mice as in vivo models, we show that deletion of hlyII strongly reduces virulence. In addition, we show that after infection of Bombyx mori larvae, the immune cells are apoptotic, demonstrating that HlyII induces apoptosis of phagocytic cells in vivo. Altogether, our results clearly unravel HlyII as a novel virulence protein that induces apoptosis in phagocytic cells in vitro and in vivo.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Guillemet, Elisabeth
(author)
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Ngo-Camus, Maud
(author)
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Clybouw, Cyril
(author)
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Puhar, Andrea,1978-Unité PMM, INSERM U786, Institut Pasteur, 75724 Paris Cedex 15, France.(Swepub:umu)anpu0007
(author)
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Moris, Arnaud
(author)
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Gohar, Michel
(author)
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Lereclus, Didier
(author)
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Ramarao, Nalini
(author)
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Unité PMM, INSERM U786, Institut Pasteur, 75724 Paris Cedex 15, France.
(creator_code:org_t)
Related titles
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In:Cellular Microbiology: Hindawi Limited13:1, s. 92-1081462-58141462-5822
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Tran, Seav-Ly
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Guillemet, Elisa ...
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Ngo-Camus, Maud
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Clybouw, Cyril
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Puhar, Andrea, 1 ...
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Moris, Arnaud
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Gohar, Michel
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Lereclus, Didier
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Ramarao, Nalini
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Immunology
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Cellular Microbi ...
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Umeå University