Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37

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Chandra, Naresh,1987-Umeå universitet,Avdelningen för virologi (author)

Sulfated Glycosaminoglycans as Viral Decoy Receptors for Human Adenovirus Type 37

Article/chapterEnglish2019

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2019-03-12
MDPI,2019
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:umu-158515
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-158515URI
https://doi.org/10.3390/v11030247DOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Glycans on plasma membranes and in secretions play important roles in infection by many viruses. Species D human adenovirus type 37 (HAdV-D37) is a major cause of epidemic keratoconjunctivitis (EKC) and infects target cells by interacting with sialic acid (SA)-containing glycans via the fiber knob domain of the viral fiber protein. HAdV-D37 also interacts with sulfated glycosaminoglycans (GAGs), but the outcome of this interaction remains unknown. Here, we investigated the molecular requirements of HAdV-D37 fiber knob:GAG interactions using a GAG microarray and demonstrated that fiber knob interacts with a broad range of sulfated GAGs. These interactions were corroborated in cell-based assays and by surface plasmon resonance analysis. Removal of heparan sulfate (HS) and sulfate groups from human corneal epithelial (HCE) cells by heparinase III and sodium chlorate treatments, respectively, reduced HAdV-D37 binding to cells. Remarkably, removal of HS by heparinase III enhanced the virus infection. Our results suggest that interaction of HAdV-D37 with sulfated GAGs in secretions and on plasma membranes prevents/delays the virus binding to SA-containing receptors and inhibits subsequent infection. We also found abundant HS in the basement membrane of the human corneal epithelium, which may act as a barrier to sub-epithelial infection. Collectively, our findings provide novel insights into the role of GAGs as viral decoy receptors and highlight the therapeutic potential of GAGs and/or GAG-mimetics in HAdV-D37 infection.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
adenovirus
antiviral drugs
cellular receptor
decoy receptor
epidemic keratoconjunctivitis
glycosaminoglycan
tropism

Added entries (persons, corporate bodies, meetings, titles ...)

Liu, Yan (author)
Liu, Jing-XiaUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Oftalmiatrik(Swepub:umu)liji0001 (author)
Frängsmyr, LarsUmeå universitet,Avdelningen för virologi(Swepub:umu)lacfrr79 (author)
Wu, Nian (author)
Silva, Lisete M (author)
Lindström, Mona,1961-Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Oftalmiatrik(Swepub:umu)moli0003 (author)
Chai, Wengang (author)
Domellöf, Fatima PedrosaUmeå universitet,Institutionen för integrativ medicinsk biologi (IMB),Oftalmiatrik(Swepub:umu)fape0001 (author)
Feizi, Ten (author)
Arnberg, Niklas,1987-Umeå universitet,Avdelningen för virologi(Swepub:umu)niar0001 (author)
Umeå universitetAvdelningen för virologi (creator_code:org_t)

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In:Viruses: MDPI11:31999-4915

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By the author/editor: Chandra, Naresh, ...; Liu, Yan; Liu, Jing-Xia; Frängsmyr, Lars; Wu, Nian; Silva, Lisete M; show more...; Lindström, Mona, ...; Chai, Wengang; Domellöf, Fatima ...; Feizi, Ten; Arnberg, Niklas, ...; show less...

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By the university: Umeå University

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