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Nuclear processing ...
Nuclear processing of nascent transcripts determines synthesis of full-length proteins and antigenic peptides
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Martins, Rodrigo Prado (author)
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Malbert-Colas, Laurence (author)
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Lista, Maria Jose (author)
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Daskalogianni, Chrysoula (author)
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Apcher, Sebastien (author)
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Pla, Marika (author)
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Findakly, Sarah (author)
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Blondel, Marc (author)
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- Fåhraeus, Robin (author)
- Umeå universitet,Patologi,Universite Paris 7, Paris, France; ICCVS, University of Gdansk, Science, Gdansk, Poland; RECAMO, Masaryk Memorial Cancer Institute, Brno, Czech Republic
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(creator_code:org_t)
- 2019-01-09
- 2019
- English.
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In: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 47:6, s. 3086-3100
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Abstract
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- Peptides presented on major histocompatibility (MHC) class I molecules form an essential part of the immune system's capacity to detect virus-infected or transformed cells. Earlier works have shown that pioneer translation peptides (PTPs) for the MHC class I pathway are as efficiently produced from introns as from exons, or from mRNAs targeted for the nonsense-mediated decay pathway. The production of PTPs is a target for viral immune evasion but the underlying molecular mechanisms that govern this non-canonical translation are unknown. Here, we have used different approaches to show how events taking place on the nascent transcript control the synthesis of PTPs and full-length proteins. By controlling the subcellular interaction between the G-quadruplex structure (G4) of a gly-ala encoding mRNA and nucleolin (NCL) and by interfering with mRNA maturation using multiple approaches, we demonstrate that antigenic peptides derive from a nuclear non-canonical translation event that is independently regulated from the synthesis of full-length proteins. Moreover, we show that G4 are exploited to control mRNA localization and translation by distinguishable mechanisms that are targets for viral immune evasion.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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