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A role for the Sacc...
A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling
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- Kasari, Villu (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS)
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Pochopien, Agnieszka A. (author)
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- Margus, Tonu (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS)
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- Murina, Victoriia (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS)
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- Turnbull, Kathryn Jane (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS)
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- Zhou, Yang, 1986- (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Nissan, Tracy (author)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,University of Sussex, UK
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Graf, Michael (author)
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Novacek, Jiri (author)
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- Atkinson, Gemma C. (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Johansson, Marcus J. O. (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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Wilson, Daniel N. (author)
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- Hauryliuk, Vasili, 1980- (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Molekylär Infektionsmedicin, Sverige (MIMS),University of Tartu, Institute of Technology, 50411 Tartu, Estonia
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(creator_code:org_t)
- 2019-07-12
- 2019
- English.
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In: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 47:16, s. 8807-8820
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Abstract
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- Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ2) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3′-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Kasari, Villu
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Pochopien, Agnie ...
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Margus, Tonu
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Murina, Victorii ...
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Turnbull, Kathry ...
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Zhou, Yang, 1986 ...
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show more...
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Nissan, Tracy
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Graf, Michael
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Novacek, Jiri
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Atkinson, Gemma ...
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Johansson, Marcu ...
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Wilson, Daniel N ...
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Hauryliuk, Vasil ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
- Articles in the publication
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Nucleic Acids Re ...
- By the university
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Umeå University
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Stockholm University