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Evaluation of growt...
Evaluation of growth, stemness, and angiogenic properties of dental pulp stem cells cultured in cGMP xeno-/serum-free medium
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- Qu, Chengjuan, 1967- (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Brohlin, Maria, 1966- (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Division of Clinical Immunology and Transfusion Medicine, Tissue Establishment, Cell Therapy Unit, Department of Laboratory Medicine, Umeå University Hospital, Umeå, Sweden
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- Kingham, Paul J. (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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- Kelk, Peyman (author)
- Umeå universitet,Institutionen för integrativ medicinsk biologi (IMB)
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(creator_code:org_t)
- 2019-12-30
- 2020
- English.
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In: Cell and Tissue Research. - : Springer. - 0302-766X .- 1432-0878. ; 380, s. 93-105
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Abstract
Subject headings
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- This study was aimed to investigate the effects of cGMP xeno-/serum-free medium (XSF, Irvine Scientific) on the properties of human dental pulp stem cells (DPSCs). DPSCs, from passage 2, were cultured in XSF or fetal bovine serum (FBS)-supplemented medium, and sub-cultured up to passage 8. Cumulative population doublings (PDs) and the number of colony-forming-units (CFUs) were determined. qRT-PCR, ELISA, and in vitro assays were used to assess angiogenic capacity. Flow cytometry was used to measure CD73, CD90, and CD105 expression. Differentiation into osteo-, adipo-, and chondrogenic cell lineages was performed. DPSCs showed more elongated morphology, a reduced rate of proliferation at later passages, and lower CFU counts in XSF compared with FBS. Expression of angiogenic factors at the gene and protein levels varied in the two media and with passage number, but cells grown in XSF had more in vitro angiogenic activity. The majority of early and late passage DPSCs cultured in XSF expressed CD73 and CD90. In contrast, the percentage of CD105 positive DPSCs in XSF medium was significantly lower with increased passage whereas the majority of cells cultured in FBS were CD105 positive. Switching XSF-cultured DPSCs to medium supplemented with human serum restored the expression of CD105. The tri-lineage differentiation of DPSCs cultured under XSF and FBS conditions was similar. We showed that despite reduced CD105 expression levels, DPSCs expanded in XSF medium maintained a functional MSC phenotype. Furthermore, restoration of CD105 expression is likely to occur upon in vivo transplantation, when cells are exposed to human serum.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- CD105
- cGMP xenogeneic serum-free culture
- Human dental pulp stem cells
- Mesenchymal stromal cell culture
Publication and Content Type
- ref (subject category)
- art (subject category)
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