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Diet-dependent depl...
Diet-dependent depletion of queuosine in tRNAs in Caenorhabditis elegans does not lead to a developmental block.
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Gaur, Rahul (author)
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- Björk, Glenn (author)
- Umeå universitet,Institutionen för molekylärbiologi (Teknisk-naturvetenskaplig fakultet),Björk
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- Tuck, Simon (author)
- Umeå universitet,Umeå centrum för molekylär patogenes (UCMP)
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Varshney, Umesh (author)
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(creator_code:org_t)
- 2007
- 2007
- English.
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In: J Biosci. - 0250-5991. ; 32:4, s. 747-54
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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Abstract
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- Queuosine (Q), a hypermodified nucleoside,occurs at the wobble position of transfer RNAs (tRNAs)with GUN anticodons. In eubacteria, absence of Q affects messenger RNA (mRNA) translation and reduces the virulence of certain pathogenic strains. In animal cells,changes in the abundance of Q have been shown to correlate with diverse phenomena including stress tolerance, cell proliferation and tumour growth but the function of Q in animals is poorly understood. Animals are thought to obtain Q (or its analogues) as a micronutrient from dietary sources such as gut micro flora. However,the difficulty of maintaining animals under bacteria-free conditions on Q-deficient diets has severely hampered the study of Q metabolism and function in animals. In this study,we show that as in higher animals, tRNAs in the nematode Caenorhabditis elegans are modified by Q and its sugar derivatives. When the worms were fed on Q-deficient Escherichia coli, Q modification was absent from the worm tRNAs suggesting that C.elegans lacks a de novo pathway of Q biosynthesis. The inherent advantages of C.elegans as a model organism, and the simplicity of conferring a Q-deficient phenotype on it make it an ideal system to investigate the function of Q modification in tRNA.
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